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Angiopoietin 2 (ANGPT2) (N-Term), (AA 21-40) antibody

Details for Product No. ABIN118023
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Antigen
Synonyms ang2, angpt2, ANG-2, ANGPT2, Agpt2, Ang-2, Ang2, AGPT2, ANG2, ANGIOPOIETIN-2
Epitope
N-Term, AA 21-40
(9), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(40), (33), (29), (13), (12), (12), (1), (1), (1), (1)
Host
Rabbit
(52), (3), (1), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(7), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(47), (26), (21), (14), (13), (11), (10), (3), (1), (1)
Pubmed 2 references available
Quantity 0.2 mL
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Catalog No. ABIN118023
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Immunogen This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide, corresponding to a region (aa 21-40) near the N-terminus of mouse angiopoietin 2 protein conjugated to KLH using maleimide.
Sequence N-N-F-R-K-S-V-D-S-T-G-R-R-Q-Y-Q-V-Q-N-G-P Remarks: A residue of cysteine was added to the amino terminal end to facilitate coupling.
Specificity This antiserum is directed against mouse angiopoietin 2 and shows no reactivity with angiopoietin 1 on mouse derived proteins. A 75 kDa band is detected when assayed by Immunoblot. This reagent cross reacts with human angiopoietin 2. Partially cross reactivity is noted with human angiopoietin 1.
Purification Serum
Alternative Name Angiopoietin-2
Background Angiopoeitin-1 (Ang-1) and Antiopoietin-2 (Ang2) are important for development of the endothelium, by regulating tyrosine phosphorylation of the membrane receptor Tie-2/Tek. Ang-1 binding to Tie-2/Tek causes phosphorylation of the receptor. Ang-2 competes for this binding, and thus blocks receptor phosphorylation. Ang-2 expression occurs at sites of vascular remodelling: dorsal aorta and major aortic branches, ovary, placenta and uterus. Anti-Ang 1 and anti-Ang 2 antibodies recognize a band above 55 kDa. Both antibodies show banding at 75 kDa which resembles the original size, because these are soluble highly glycosylated proteins, which should run higher that the calculated molecular weight of 55 kD.
Alternate names: ANG-2, ANG2, ANGPT2
Gene ID 11601
NCBI Accession NP_031452.2
UniProt O35608
Research Area Growth Factors
Application Notes Suitable for Western blotting (1: 500) and other antibody based assays. The reaction of this antiserum directly with cell supernatants may result in highbackground due to reactivity of components in the serum. This can be alleviated by firstimmunoprecipitating the antibody: antigen complex and then detecting the antigen. Thismethod results in very clean and strong signal. Recommended Dilutions: This product was assayed by immunoblot against supernatantsof angiopoietin expressing endothelial cells from human and mouse sources. A singleband of the expected apparent molecular weight was observed at a 1: 500 dilution. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 85.0 mg/mL (by refractometry)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 0.09% (w/v) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Supplier Images
anti-Angiopoietin 2 (ANGPT2) (N-Term), (AA 21-40) antibody Immunoblotting. Rabbit anti-Ang 2 was used at a.,.1:500 dilution to detect mouse Ang 2 by western blot found.,.in supernatants of mouse-angiopoietin-expressing.,.endothelial cells. Lane 1 - mock endothelial cells..,.Lane 2 -mouse Ang-2 (clone 2-9) expressing cells..,.Lane 3 – mouse Ang-1 (clone 1-15) expressing cells..,.Lane 4 - mouse Ang-1(clone 1-8) expressing cells..,.Lane 5 - wt endothelial cells..,.Approximately 20 ug of each lysate was loaded on a 10%.,.SDS-PAGE. Immunoprecipitation preceded reaction with.,.primary antibody at room temperature for 1 h. After.,.subsequent washing, a 1:5,000 dilution of HRP conjugated.,.Gt-a-Rabbit IgG preceded color development.
General Suri, Jones, Patan et al.: "Requisite role of angiopoietin-1, a ligand for the TIE2 receptor, during embryonic angiogenesis." in: Cell, Vol. 87, Issue 7, pp. 1171-80, 1997 (PubMed).

Maisonpierre, Suri, Jones et al.: "Angiopoietin-2, a natural antagonist for Tie2 that disrupts in vivo angiogenesis." in: Science (New York, N.Y.), Vol. 277, Issue 5322, pp. 55-60, 1997 (PubMed).

Validation Images
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