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Tumor Necrosis Factor antibody (TNF)

Details for Product anti-TNF Antibody No. ABIN118025, Supplier: Log in to see
Antigen
  • TNF
  • tnf
  • dif
  • tnfa
  • xtnf
  • TNF-a
  • tnfsf2
  • tnf-alpha
  • TNF-alpha
  • DIF
  • TNFA
  • TNFSF2
  • TNFalpha
  • Tnfa
  • Tnfsf1a
  • RATTNF
  • TNFa
  • cTNF
  • TNF-ALPHA
  • Cachectin
  • Tnf-alpha
  • tnfa-like
Alternatives
anti-Mouse (Murine) Tumor Necrosis Factor antibody for ELISA
Reactivity
Mouse (Murine)
701
307
142
55
49
32
27
27
23
18
12
12
8
7
5
4
4
4
3
3
2
2
1
1
1
Host
Rabbit
532
395
157
43
13
9
9
7
4
1
Clonality
Polyclonal
Conjugate
This Tumor Necrosis Factor antibody is un-conjugated
116
67
35
21
18
9
9
7
5
4
4
4
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Radioimmunoassay (RIA), Western Blotting (WB)
627
584
315
208
120
100
92
85
75
62
56
52
25
24
24
22
15
14
8
8
6
6
5
5
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
Supplier
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Immunogen Recombinant mouse TNFalpha produced in E. coli
Purification Serum
Alternative Name Tumor Necrosis Factor (TNF-Alpha) (TNF Antibody Abstract)
Background Synonyms: Cachectin, TNF, TNF-a, TNFA, TNFSF2, Tumor necrosis factor ligand superfamily member 2
Gene ID 10090
UniProt P06804
Research Area Cytokines, Growth Factors, Receptors, Immunology, Inflammation, Cancer, Virology
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis
Application Notes Suitable for use in : Immunoblot: Using HRP Goat-anti-Rabbit IgG [H&L] and TMB as a substrate. A workingdilution range of 1: 400-1: 800 is suggested for this application to detect TNFα fromsupernatants or lysates of 2 x 10^6 endotoxin-stimulated mouse peripheral bloodmononuclear cells (PBMC). PBMC are stimulated for 24 hours with 1 % (v/v) mouse serumplus 10 ng/mL E. coli LPS. Immunohistochemistry: A dilution range of 1: 400 is suggested for this immunoassay. Either paraffin fixation or cryofixation can be used for immunohistochemistry using adilution of 1: 200 for staining of TNFα. Immunoprecipitation: A dilution range of 1: 400-1: 800 is suggested for this immunoassay. For immunoprecipitation, pre-clearing with a non-specific rabbit IgG is helpful to reducebackground. ELISA: Using HRP Conjugated Anti-Rabbit IgG [H&L] (Goat) and ABTS as a substrate for 30 minutes at room temperature. A working dilution range of 1: 1,000 to 1: 2,000 is suggestedfor this product. For use in ELISA formats, this antibody is best used as the secondantibody in combination with a monoclonal antibody as a capture antibody. Radioimmunoassay: A dilution of 1: 8,000 is suggested. Neutralization
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 85 mg/mL (by refractometry)
Handling Advice Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
Storage -20 °C
Storage Comment Store vial at -20° C or below prior to opening. For extended storage aliquot contents and freeze at -20 °C or below.
Background publications Birkas Kovats, Palik, Faludi et al.: "[Possible connection between ghrelin, resistin and TNF-alpha levels and the metabolic syndrome caused by atypical antipsychotics]" in: Neuropsychopharmacologia Hungarica : a Magyar Pszichofarmakológiai Egyesület lapja = official journal of the Hungarian Association of Psychopharmacology, Vol. 7, Issue 3, pp. 132-9, 2006 (PubMed).

Nedwin, Naylor, Sakaguchi et al.: "Human lymphotoxin and tumor necrosis factor genes: structure, homology and chromosomal localization." in: Nucleic acids research, Vol. 13, Issue 17, pp. 6361-73, 1985 (PubMed).

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