Tumor Necrosis Factor (TNF) antibody

Antigen: Tumor Necrosis Factor (TNF)

Synonyms: DIF, TNFA, TNFSF2, TNF-alpha, B12, B61, EDP1, MGC2317, Edp1, Edp-1, Tnfip1, Tnfa, RATTNF, MGC124630, zgc:55302, wu:fi06b02, TNFAIP1, TNF, tnf, DKFZp468A2328, Tnfsf1a, TNFalpha, MGC151434, TNFa, cTNF, Tnf-alpha, tnfa-like, TNF-ALPHA, dif, tnfa, xtnf, TNF-a, tnfsf2, tnf-alpha, Cachectin

Clonality: Polyclonal

Host: Rabbit

Reactivity: Mouse (Murine)

Conjugate: Un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA, Functional Studies (Func), Immunoprecipitation (IP), Radioimmunoassay (RIA), Western Blotting (WB)

Catalog no.: ABIN118025

Additional Information

Alternative name: Tumor necrosis factor (TNF-alpha)

UniProt: P06804

Immunogen: Recombinant mouse TNFalpha produced in E. coli

Cross-Reactivity: Mouse (Murine)

Characteristics: Synonyms: TNF, TNF-a, TNFA, TNFSF2, Cachectin, Tumor necrosis factor ligand superfamily member 2

Synonyms: DIF, TNFA, TNFSF2, TNF-alpha, B12, B61, EDP1, MGC2317, Edp1, Edp-1, Tnfip1, Tnfa, RATTNF, MGC124630, zgc:55302, wu:fi06b02, TNFAIP1, TNF, tnf, DKFZp468A2328, Tnfsf1a, TNFalpha, MGC151434, TNFa, cTNF, Tnf-alpha, tnfa-like, TNF-ALPHA, dif, tnfa, xtnf, TNF-a, tnfsf2, tnf-alpha, Cachectin

Application Details

Application Notes: Suitable for use in : Immunoblot: Using HRP Goat-anti-Rabbit IgG [H&L] and TMB as a substrate. A workingdilution range of 1: 400-1: 800 is suggested for this application to detect TNFα fromsupernatants or lysates of 2 x 10e6 endotoxin-stimulated mouse peripheral bloodmononuclear cells (PBMC). PBMC are stimulated for 24 hours with 1% (v/v) mouse serumplus 10 ng/mL E. coli LPS. Immunohistochemistry: A dilution range of 1: 400 is suggested for this immunoassay. Either paraffin fixation or cryofixation can be used for immunohistochemistry using adilution of 1: 200 for staining of TNFα. Immunoprecipitation: A dilution range of 1: 400-1: 800 is suggested for this immunoassay. For immunoprecipitation, pre-clearing with a non-specific rabbit IgG is helpful to reducebackground. ELISA: Using HRP Conjugated Anti-Rabbit IgG [H&L] (Goat) and ABTS as a substrate for 30minutes at room temperature. A working dilution range of 1: 1,000 to 1: 2,000 is suggestedfor this product. For use in ELISA formats, this antibody is best used as the secondantibody in combination with a monoclonal antibody as a capture antibody. Radioimmunoassay: A dilution of 1: 8,000 is suggested. NeutralizationOther applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 85 mg/ml (by refractometry)

Purification: Serum

Storage: Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extendedstorage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Shelf life: one year from despatch.

Research Area: Cytokines, Growth Factors, Receptors, Immunology, Inflammation, Cancer, Virology

Restrictions: For Research Use only

Publications

Nedwin, Naylor, Sakaguchi et al.: "Human lymphotoxin and tumor necrosis factor genes: structure, homology and chromosomal localization." in: Nucleic acids research, Vol. 13, Issue 17, pp. 6361-73, 1985 (PubMed).

Birkas Kovats, Palik, Faludi et al.: "[Possible connection between ghrelin, resistin and TNF-alpha levels and the metabolic syndrome caused by atypical antipsychotics]" in: Neuropsychopharmacologia Hungarica : a Magyar Pszichofarmakológiai Egyesület lapja = official journal of the Hungarian Association of Psychopharmacology, Vol. 7, Issue 3, pp. 132-9, 2006 (PubMed).