IRS1 antibody (pSer307)

Catalog No. ABIN118052

Product Details anti-IRS1 Antibody

Target: IRS1 (Insulin Receptor Substrate 1 (IRS1))

Binding Specificity: AA 298-316, pSer307

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IRS1 antibody is un-conjugated

Application: Enzyme Immunoassay (EIA)

Sequence: Protein Sequence: Human IRS1, 1242 aa, predicted MW 131.6 kDa: 1 masppesdgf sdvrkvgylr kpksmhkrff vlraaseagg parleyyene kkwrhkssap 61 krsiplescf ninkradskn khlvalytrd ehfaiaadse aeqdswyqal lqlhnrakgh 121 hdgaaalgag ggggscsgss glgeagedls ygdvppgpaf kevwqvilkp kglgqtknli 181 giyrlcltsk tisfvklnse aaavvlqlmn irrcghsenf ffievgrsav tgpgefwmqv 241 ddsvvaqnmh etileamram sdefrprsks qsssncsnpi svplrrhhln npppsqvglt 301 rrsrtesita tspasmvggk pgsfrvrass dgegtmsrpa svdgspvsps tnrthahrhr 361 gsarlhppln hsrsipmpas rcspsatspv slsssstsgh gstsdclfpr rssasvsgsp 421 sdggfissde ygsspcdfrs sfrsvtpdsl ghtppargee elsnyicmgg kgpstltapn 481 ghyilsrggn ghrctpgtgl gtspalagde aasaadldnr frkrthsagt sptithqktp 541 sqssvasiee ytemmpaypp gggsggrlpg hrhsafvptr sypeeglemh plerrgghhr 601 pdsstlhtdd gympmspgva pvpsgrkgsg dympmspksv sapqqiinpi rrhpqrvdpn 661 gymmmspsgg cspdigggps ssssssnavp sgtsygklwt ngvgghhshv lphpkppves 721 sggkllpctg dymnmspvgd sntsspsdcy ygpedpqhkp vlsyyslprs fkhtqrpgep 781 eegarhqhlr lstssgrlly aataddssss tssdslgggy cgarlepslp hphhqvlqph 841 lprkvdtaaq tnsrlarptr lslgdpkast lprareqqqq qqpllhppep kspgeyvnie 901 fgsdqsgyls gpvafhssps vrcpsqlqpa preeetgtee ymkmdlgpgr raawqestgv 961 emgrlgpapp gaasicrptr avpssrgdym tmqmscprqs yvdtspaapv syadmrtgia 1021 aeevslprat maaassssaa sasptgpqga aelaahssll ggpqgpggms aftrvnlspn 1081 rnqsakvira dpqgcrrrhs setfsstpsa trvgntvpfg agaavggggg sssssedvkr 1141 hssasfenvw lrpgelggap kepaklcgaa gglenglnyi dldlvkdfkq cpqectpepq 1201 ppppppphqp lgsgessstr rssedlsaya sisfqkqped rq

Specificity: This affinity-purified antibody is directed against the phosphorylated form of human IRS1 protein at the pS307 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human IRS1 pS307 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human IRS1 is minimal by ELISA. A BLAST analysis was used to suggest cross reactivity with IRS1 from human, mouse, rat, dog and vervet monkey based on 100 % homology with the immunizing sequence. Partial reactivity is also expected against IRS1 from pig (94 %), bovine (94 %) and chicken (88 %) sources. Reactivity of this antibody with IRS1 from other species is unknown.

Purification: Immunoaffinity chromatography.

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 298-316 of human IRS1 protein.

Application Details

Application Notes: This affinity purified antibody has been tested for use in ELISA (1: 2,000-1: 10,000) andwestern blot (1: 250-1: 1,500). Specific conditions for reactivity should be optimized by theend user. Expect a band approximately 180 kDa in size corresponding to phosphorylatedIRS1 protein by western blotting in the appropriate cell lysate or extract. Minimal reactivityis observed against the non-phosphorylated form of the immunizing peptide. This antibodyis phospho specific for pS307 of IRS1 protein.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C

Storage Comment: Store undiluted at 2-8 °Cfor one month or (in aliquots) at -20 °C for longer.

Target Details for IRS1

Target: IRS1 (Insulin Receptor Substrate 1 (IRS1))

Alternative Name: IRS1 (IRS1 Products)

Synonyms: HIRS-1 antibody, G972R antibody, IRS-1 antibody, IRS1IRM antibody, irs1 antibody, irsu antibody, IRS1 antibody, irs-1 antibody, irs1-a antibody, insulin receptor substrate 1 antibody, insulin receptor substrate 2 antibody, insulin receptor substrate 1 S homeolog antibody, IRS1 antibody, Irs1 antibody, irs2 antibody, irs1.S antibody

Background: Insulin Receptor Substrate 1 (IRS1) acts as a signaling molecule for IL-4, insulin and insulin-like growth factor-I (IGF-I) receptors. When phosphorylated by the insulin receptor, IRS1 binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. When bound, IRS1 typically activates phosphatidylinositol 3-kinase p85 subunit. IRS1 interacts with both the NPXY motif of tyrosine-phosphorylated IGF1R and the INSR through the PTB domain. Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor. Ser-307 phosphorylation is involved in the mechanism of insulin resistance in type 2 diabetes. Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM).Synonyms: IRS-1, Insulin receptor substrate 1

Gene ID: 3667

NCBI Accession: NP_005535

UniProt: P35568

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Negative Regulation of Hormone Secretion, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process