ING3 antibody (Isoform 1)

Catalog No. ABIN118056

Product Details anti-ING3 Antibody

Target: ING3 (Inhibitor of Growth Family, Member 3 (ING3))

Binding Specificity: AA 294-304, Isoform 1

Reactivity: Human, Mouse, Rat, Dog, Chicken, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This ING3 antibody is un-conjugated

Application: Enzyme Immunoassay (EIA)

Specificity: This is an affinity-purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. Reactivity occurs against human p47 ING3 protein. The sequence is not present in isoforms 2 and 3 (see below). However, 100 % homology is on record for this protein from human, dog, rat, chimpanzee and orangutan. Cross reactivity with p47 ING3 protein from mouse and chicken sources is also expected based on high homology (one amino acid change in sequence) by BLAST. Reactivity with p47 ING3 proteins from other sources is not known. Human p47 ING3 isoform 1, 418 aa, predicted MW 46.7 kDa: 1 mlyledylem ieqlpmdlrd rftemremdl qvqnamdqle qrvseffmna kknkpewree 61 qmasikkdyy kaledadekv qlanqiydlv drhlrkldqe lakfkmelea dnagiteile 121 rrsleldtps qpvnnhhahs htpvekrkyn ptshhtttdh ipekkfksea llstltsdas 181 kentlgcrnn nstassnnay nvnssqplgs ynigslssgt gagaitmaaa qavqataqmk 241 egrrtsslka syeafknndf qlgkefsmar etvgyssssa lmttltqnas ssaadsrsgr 301 ksknnnksss qqssssssss slsscsssst vvqeisqqtt vvpesdsnsq vdwtydpnep 361 rycicnqvsy gemvgcdnqd cpiewfhygc vglteapkgk wycpqctaam krrgsrhk

Purification: Immunoaffinity chromatography.

Immunogen: Synthetic peptide corresponding aa 294-304 of Human ING3 protein (Inhibitor of growth family, member 3). This sequence only shows homology to isoform 1 for ING3

Application Details

Application Notes: This affinity purified antibody has been tested for use in ELISA (1: 10,000-1: 40,000) againstthe immunizing peptide. Specific conditions for Western blotting (1: 200-1: 2,000) reactivityshould be optimized by the end user. Expect a band at approximately 47 kDa in sizecorresponding to ING3 isoform 1 by western blotting in the appropriate cell lysate orextract.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.1 mg/mL (UVabs. at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 0.01 % sodium azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Dilute only prior to immediate use. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C or below prior to opening. Aliquot contents and freeze at -20 °C or below.

Target Details for ING3

Target: ING3 (Inhibitor of Growth Family, Member 3 (ING3))

Alternative Name: ING3 (ING3 Products)

Synonyms: ING3 antibody, Eaf4 antibody, ING2 antibody, MEAF4 antibody, p47ING3 antibody, 1300013A07Rik antibody, P47ING3 antibody, zgc:56327 antibody, inhibitor of growth family member 3 antibody, inhibitor of growth family, member 3 antibody, inhibitor of growth protein 3 antibody, inhibitor of growth family member 3 L homeolog antibody, ING3 antibody, Ing3 antibody, CpipJ_CPIJ009396 antibody, ing3 antibody, ing3.L antibody

Background: P47 ING3 is a tumor suppressor protein similar to ING1 that can interact with TP53, inhibit cell growth, and induce apoptosis. This protein contains a PHD-finger, which is a common motif in proteins involved in chromatin remodeling. This gene can activate p53 trans-activated promoters, including promoters of p21/waf1 and bax. Over-expression of this gene has been shown to inhibit cell growth and induce apoptosis. Allelic loss and reduced expression of this gene were detected in head and neck cancers. Multiple alternatively spliced transcript variants have been observed. The accession number listed below is for variant (1) that encodes the longest isoform.Synonyms: HSPC301, Inhibitor of growth protein 3, p47ING3

Gene ID: 54556

NCBI Accession: NP_061944

UniProt: Q9NXR8