Goat anti-Cow (Bovine) IgG (Whole Molecule) Antibody (Biotin)

Details for Product No. ABIN118075, Supplier: Log in to see
Antigen
Epitope
Whole Molecule
5028
2577
1931
1069
1007
857
330
299
35
34
31
8
7
7
3
3
2
2
1
1
1
1
1
1
Reactivity
Cow (Bovine)
2912
2859
2405
1752
1485
796
623
441
381
380
355
340
307
293
208
148
139
114
41
29
27
25
12
11
9
8
6
5
5
5
4
4
4
4
4
4
4
4
3
3
3
2
2
1
1
1
1
1
Host
Goat
6892
4912
1878
895
703
401
92
54
52
21
9
8
5
5
2
1
1
Clonality
Polyclonal
Conjugate
Biotin
2235
1889
1739
1374
765
679
519
300
299
288
274
268
217
188
178
124
103
79
69
60
54
46
43
40
40
36
34
32
29
29
27
27
27
27
27
27
27
27
22
22
19
19
19
19
16
15
15
15
15
15
14
12
12
11
10
10
9
9
9
9
9
9
9
8
8
8
8
7
7
6
6
6
6
6
6
5
5
5
5
5
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Immunogen Bovine IgG whole molecule.
Characteristics Molar Ratio: 10-20 BAC molecules per Goat IgG molecule.
Purification Immunoaffinity chromatography.
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Bovine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Background Synonyms: Bovine Immunoglobulin G
Research Area Immunology, Secondary Antibodies
Application Notes Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well asother antibody based assays using streptavidin or avidin conjugates requiring lot-to-lotconsistency. This product has been assayed against 1.0 g of Bovine IgG in a standardcapture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1:11,000 to 1:45,000 of the reconstitution concentration is suggestedfor this product.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Reconstitution Restore with 1.0 mL of deionized water (or equivalent).
Concentration 2.0 mg/mL
Buffer 0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, pH 7.4 contaning 0.01 % (w/v) Thimerisol as preservative and 10 mg/mL Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer.
Preservative Thimerosal (Merthiolate)
Precaution of Use Biohazard Informations: This product contains thimerosal which is hazardous.
Handling Advice Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Storage 4 °C/-20 °C
Storage Comment Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50 % and then aliquot contents and freeze at -28 °C or below. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
Background publications Bayer, Wilchek: "Protein biotinylation." in: Methods in enzymology, Vol. 184, pp. 138-60, 1990 (PubMed).