Goat anti-Cow IgG (Whole Molecule) Antibody (HRP)

Catalog No. ABIN118077

Product Details

Target: IgG

Binding Specificity: Whole Molecule

Reactivity: Cow

Host: Goat

Clonality: Polyclonal

Conjugate: HRP

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Purification: Immunoaffinity Chromatography.
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Bovine IgG coupled to agarose beads.

Immunogen: Bovine IgG whole molecule.

Application Details

Application Notes: Suitable for Immunoblotting (1/1,000-1/4,000, Western or dot blot), ELISA (1/10,000-1/40,000), Immunoperoxidase Electron Microscopy and Immunohistochemistry (1/500-1/2,500) as well as other peroxidase-antibody based enzymatic assays requiringlot-to-lot consistency.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with 2.0 mL of deionized water (or equivalent).

Concentration: 0.8 mg/mL

Buffer: 0.01 M Potassium Phosphate, 0.21 M Sodium Chloride, pH 7.2 containing 15 mg/mL BSA (IgG and Protease free) as stabilizer.

Preservative: Azide free

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50 % and then aliquot contents and freeze at -28 °C or below. This product is stable for one month at 2-8 °C as an undiluted liquid.

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background: Synonyms: Bovine Immunoglobulin G