Glucose Oxidase (GOD) antibody (HRP)

Antigen: Glucose Oxidase (GOD)

Synonyms: LOC406081, GB19418

Clonality: Polyclonal

Host: Goat

Conjugate: HRP

Application: Enzyme Immunoassay (EIA), Western Blotting (WB)

Catalog no.: ABIN118091

Additional Information

Characteristics: Synonyms: Glucose oxyhydrase, Beta-D-glucose: oxygen 1-oxido-reductase, gox

Alternative name: Glucose Oxidase

Swiss-Prot: P13006

Immunogen: Glucose Oxidase [Aspergillus niger].

Description: Glucose Oxidase is widely used for the determination of glucose in body fluids and inremoving residual glucose or oxygen from foods and beverages. Glucose Oxidaseproducing moulds such as Aspergillus and Penicillium species are used for the biologicalproduction of gluconic acid. CATALYTIC ACTIVITY: Beta D glucose + O2 = D glucono 1,5 lactone + H2O2.

Specificity: This product is an IgG fraction antibody purified from monospecific antiserum. Assay byimmunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase,anti-Goat Serum as well as purified and partially purified Glucose Oxidase [Aspergillusniger].

Application Details

Application Notes: Suitable for use in Immunoblotting (1/1,000-1/5,000, Western or dot blot), ELISA(1/4,000-1/20,000), Immunoperoxidase Electron Microscopy and Immunohistochemistryas well as other peroxidase-antibody based enzymatic assays. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 10 mg/ml (OD280)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01%(w/v) Gentamicin Sulfate as preservative.Label: Horseradish Peroxidase (HRP)Reconstitution: Restore with

Storage: Store vial at 2-8°C prior to restoration. For extended storage reconstitute product with 50%glycerol instead of water and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature. This productis stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid cycles of freezing and thawing. Shelf life: one year from despatch.

Research Area: Signaling, Metabolism, Enzymes

Restrictions: For Research Use only

Publications

Publications: Farr, Nakane: "Immunohistochemistry with enzyme labeled antibodies: a brief review." in: Journal of immunological methods, Vol. 47, Issue 2, pp. 129-44, 1982 (PubMed).