Integrin alpha M (ITGAM) antibody (FITC)

Antigen: Integrin alpha M (ITGAM)

Synonyms: CR3A, MO1A, CD11B, MAC-1, MAC1A, SLEB6, MGC117044, CR3, MAC1, Cd11b, Ly-40, Mac-1, Mac-1a, CD11b/CD18, F730045J24Rik, ITGAM, Itgam

Clonality: Monoclonal (M1-70-15)

Host: Rat

Reactivity: Human, Mouse (Murine), Rabbit

Conjugate: FITC

Application: Flow Cytometry (FACS)

Catalog no.: ABIN118144

Additional Information

Alternative name: CD11b / ITGAM

Swiss-Prot: P05555

Immunogen: T cell enriched splenocytes from B10 mice. Spleen cells from immunised DA rats were fused with cells of the mouse NS1/1.Ag4.1 myeloma cell line

Isotype: IgG2b

Clone: M1-70-15

Description: MAC-1 is a differentiation antigen expressed by granulocytes, monocytes, NK cells and tissue macrophages. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Alternate Names: INTEGRIN ALPHA M CHAIN, MAC-1.

Specificity: This antibody recognises the CD11b cell surface antigen (also known as the alpha M integrin chain or MAC-1). Clone M1/70.15 has been reported to block iC3b binding to its receptor. ABIN118139 is recommended for this purpose. Clone M1/70.15 has been reported to as being suitable for use on PLP fixed paraffin embedded tissue but has not been tested for use on formalin fixed tissue.

Application Details

Application Notes: Flow Cytometry: 1/100, Use 10ul of the suggested working dilution to label 10e6 cells in 100ul, The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Buffer: PBS, pH7.4 containing 0.09% Sodium Azide and 1% Bovine Serum Albumin

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light. Shelf Life: one year from despatch.

Research Area: CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Publications

Publications: Springer, Galfre, Secher et al.: "Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens." in: European journal of immunology, Vol. 8, Issue 8, pp. 539-51, 1978 (PubMed).

Springer, Galfre, Secher et al.: "Mac-1: a macrophage differentiation antigen identified by monoclonal antibody." in: European journal of immunology, Vol. 9, Issue 4, pp. 301-6, 1979 (PubMed).

Beller, Springer, Schreiber: "Anti-Mac-1 selectively inhibits the mouse and human type three complement receptor." in: The Journal of experimental medicine, Vol. 156, Issue 4, pp. 1000-9, 1983 (PubMed).

Whiteland, Nicholls, Shimeld et al.: "Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 43, Issue 3, pp. 313-20, 1995 (PubMed).