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CD19 antibody (PE)

CD19 Reactivity: Mouse FACS Host: Rat Monoclonal 6D5 PE
Catalog No. ABIN118162
  • Target See all CD19 Antibodies
    CD19 (CD19 Molecule (CD19))
    Reactivity
    • 294
    • 162
    • 48
    • 30
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    • 10
    • 7
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    • 3
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    Mouse
    Host
    • 172
    • 141
    • 47
    • 4
    Rat
    Clonality
    • 232
    • 128
    • 2
    Monoclonal
    Conjugate
    • 113
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    • 1
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    This CD19 antibody is conjugated to PE
    Application
    • 263
    • 117
    • 72
    • 62
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    Flow Cytometry (FACS)
    Specificity
    This antibody recognises the CD19 cell surface antigen, a 95 kDa glycoprotein expressed by B lymphocytes. Clone 6D5 has been shown to recognise the same epitope as clone ID3 in cross-competition assays.
    Purification
    Affinity chromatography on Protein G
    Immunogen
    Human K562 cell line transfected with murine CD19. Spleen cells from immunised rats were fused with cells of the P3X63.Ag8.653 myeloma cell line.
    Clone
    6D5
    Isotype
    IgG2a
    Top Product
    Discover our top product CD19 Primary Antibody
  • Application Notes
    Flow Cytometry: Use 10 μL of Neat-1/10 diluted antibody to label 10^6 cells in 100 μL. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Reconstitution
    Restore with 1 mL distilled water
    Buffer
    PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    This product is photosensitive and should be protected from light
    Storage
    4 °C
    Storage Comment
    Prior to and following reconstitution store the antibody at 2-8 °C. DO NOT FREEZE!
  • Target
    CD19 (CD19 Molecule (CD19))
    Alternative Name
    CD19 (CD19 Products)
    Synonyms
    B4 antibody, CVID3 antibody, AW495831 antibody, CD19 antibody, CD19 molecule antibody, CD19 antigen antibody, CD19 antibody, Cd19 antibody
    Background
    CD19 is a member of the immunoglobulin superfamily and has two Ig like domains. The CD19 Molecule is expressed on 100 % of the peripheral B cells as defined by expression of kappa or lamda light chains. It is expressed on approximately 10 % of normal human peripheral blood cells and approximately 60 % of splenic lymphocytes. It is not expressed on granulocytes, monocytes or T cells as defined by CD3 expression. CD19 defines a pan B antigen which is expressed from the earliest stages of B progenitor development, but is lost on terminal differentiation to plasma cells. It may also be present on some early myeloid progenitors, particularly those of the monoblastic type. The CD19 antigen is expressed on approximately 12 % of peripheral blood lymphocytes. It appears to be expressed on myeloid leukemia cells, particularly those of monocytic lineage. Leukemia phenotype studies have demonstrated that the earliest and broadest B cell restricted antigen is the CD19 antigen.Synonyms: B-cell marker, B-lymphocyte surface antigen B4, Differentiation antigen CD19, Leu-12
    Gene ID
    12478
    NCBI Accession
    NP_033974
    UniProt
    P25918
    Pathways
    Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
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