Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) antibody (FITC)

Details for Product No. ABIN118213
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Antigen
Synonyms PTPRC, si:dkeyp-47c5.1, B220, CD45, CD45R, GP180, L-CA, LCA, LY5, T200, Cd45, Ly-5, Lyt-4, loc, Lca, RT7, cd45
Reactivity
Cat (Feline), Human, Mouse (Murine)
(652), (472), (136), (55), (40), (36), (31), (19), (19), (7), (5), (3), (3), (2), (2), (1), (1)
Host
Rat
(680), (445), (143), (2), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
FITC
(183), (121), (109), (60), (33), (19), (13), (12), (10), (9), (8), (8), (8), (8), (7), (6), (6), (6), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Flow Cytometry (FACS)
(1005), (214), (210), (201), (182), (148), (98), (94), (76), (40), (16), (8), (4), (2), (2), (2), (1), (1)
Pubmed 8 references available
Quantity 0.1 mg
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Catalog No. ABIN118213
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Immunogen Mouse pre-B tumour cells (RAW112).
Clone RA3-6B2
Isotype IgG2a
Specificity This antibody reacts with CD45R a form of the CD45 antigen expressed by B cells and lytically active subsets of NK cells and non-MHC restricted CTLs. Clone RA3-6B2 Immunoprecipitates the high molecular weight form of CD45 (220kD).
Purification Affinity Chromatography on Protein G
Alternative Name CD45 / LCA
Background CD45R is a member of the protein tyrosine phosphatase (PTP) family and a major cell surface glycoprotein. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. CD45R represents a restricted form of the CD45 family which primarily recognizes only cells of B lineage from proB cell through mature B lymphocytes and, prior to the availability of anti CD19 MAbs, was commonly used as a pan B cell marker. It also reacts with certain activated T cells, as well as non MHC restricted lytically active lymphokine activated killer (LAK) cells. CD45R contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. It is specifically expressed in hematopoietic cells and has been shown to be an essential regulator of T and B cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. CD45R also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported.
Alternate names: L-CA, Leukocyte common antigen, PTPRC, Receptor-type tyrosine-protein phosphatase C, T200
Gene ID 10090
UniProt P06800
Application Notes Flow Cytometry: Use 10 ul of neat antibody to label 10e6 cells in 100 ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.1 mg/mL
Buffer PBS, pH 7.4 containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. Should it contain a precipitate we recommend microcentrifugation before use.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Supplier Images
anti-Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) antibody (FITC) Staining of mouse spleen cells with RAT ANTI MOUSE CD45R:FITC
Background publications Coffman: "Surface antigen expression and immunoglobulin gene rearrangement during mouse pre-B cell development." in: Immunological reviews, Vol. 69, pp. 5-23, 1983 (PubMed).

Whiteland, Nicholls, Shimeld et al.: "Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 43, Issue 3, pp. 313-20, 1995 (PubMed).

Monteith, Chelack, Davis et al.: "Identification of monoclonal antibodies for immunohistochemical staining of feline B lymphocytes in frozen and formalin-fixed paraffin-embedded tissues." in: Canadian journal of veterinary research = Revue canadienne de recherche vétérinaire, Vol. 60, Issue 3, pp. 193-8, 1996 (PubMed).

Rosmalen, Martin, Dobbs et al.: "Subsets of macrophages and dendritic cells in nonobese diabetic mouse pancreatic inflammatory infiltrates: correlation with the development of diabetes." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 80, Issue 1, pp. 23-30, 2000 (PubMed).

General Spangrude, Muller-Sieburg, Heimfeld et al.: "Two rare populations of mouse Thy-1lo bone marrow cells repopulate the thymus." in: The Journal of experimental medicine, Vol. 167, Issue 5, pp. 1671-83, 1988 (PubMed).

Spangrude, Heimfeld, Weissman: "Purification and characterization of mouse hematopoietic stem cells." in: Science (New York, N.Y.), Vol. 241, Issue 4861, pp. 58-62, 1988 (PubMed).

Holmes, Langdon, Fredrickson et al.: "Analysis of neoplasms induced by Cas-Br-M MuLV tumor extracts." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 137, Issue 2, pp. 679-88, 1986 (PubMed).

Whitlock, Tidmarsh, Muller-Sieburg et al.: "Bone marrow stromal cell lines with lymphopoietic activity express high levels of a pre-B neoplasia-associated molecule." in: Cell, Vol. 48, Issue 6, pp. 1009-21, 1987 (PubMed).

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