Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) antibody (FITC)

Antigen: Protein tyrosine Phosphatase, Receptor Type, C (PTPRC)

Synonyms: LCA, LY5, B220, CD45, T200, CD45R, GP180, Lca, RT7, L-CA, PTPRC, si:dkeyp-47c5.1

Clonality: Monoclonal (RA3-6B2)

Host: Rat

Reactivity: Cat (Feline), Human, Mouse (Murine)

Conjugate: FITC

Application: Flow Cytometry (FACS)

Catalog no.: ABIN118213

Additional Information

Alternative name: CD45 / LCA

UniProt: P06800

Immunogen: Mouse pre-B tumour cells (RAW112)

Cross-Reactivity: Cat (Feline), Human, Mouse (Murine)

Isotype: IgG2a

Clone: RA3-6B2

Description: CD45R is a member of the protein tyrosine phosphatase (PTP) family and a major cell surface glycoprotein. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. CD45R represents a restricted form of the CD45 family which primarily recognizes only cells of B lineage from proB cell through mature B lymphocytes and, prior to the availability of anti CD19 MAbs, was commonly used as a pan B cell marker. It also reacts with certain activated T cells, as well as non MHC restricted lytically active lymphokine activated killer (LAK) cells. CD45R contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. It is specifically expressed in hematopoietic cells and has been shown to be an essential regulator of T and B cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. CD45R also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. Alternate Names: Lymphocyte Antigen 5, Leukocyte Common Antigen, B220, LY-5, L-CA, T200, Ptprc.

Application Details

Application Notes: Flow Cytometry: Use 10 ul of neat antibody to label 10e6 cells in 100 ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.1 mg/ml

Buffer: PBS, pH 7.4 containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light. Shelf Life: one year from despatch.

Restrictions: For Research Use only

Images

Staining of mouse spleen cells with RAT ANTI MOUSE CD45R:FITC

Publications

Spangrude, Muller-Sieburg, Heimfeld et al.: "Two rare populations of mouse Thy-1lo bone marrow cells repopulate the thymus." in: The Journal of experimental medicine, Vol. 167, Issue 5, pp. 1671-83, 1988 (PubMed).

Spangrude, Heimfeld, Weissman: "Purification and characterization of mouse hematopoietic stem cells." in: Science (New York, N.Y.), Vol. 241, Issue 4861, pp. 58-62, 1988 (PubMed).

Holmes, Langdon, Fredrickson et al.: "Analysis of neoplasms induced by Cas-Br-M MuLV tumor extracts." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 137, Issue 2, pp. 679-88, 1986 (PubMed).

Whitlock, Tidmarsh, Muller-Sieburg et al.: "Bone marrow stromal cell lines with lymphopoietic activity express high levels of a pre-B neoplasia-associated molecule." in: Cell, Vol. 48, Issue 6, pp. 1009-21, 1987 (PubMed).