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CD45 antibody (Protein tyrosine Phosphatase, Receptor Type, C)

Details for Product anti-PTPRC Antibody No. ABIN118214, Supplier: Log in to see
Antigen
  • B220
  • CD45
  • Cd45
  • cd45
  • CD45R
  • GP180
  • L-CA
  • Lca
  • LCA
  • loc
  • Ly-5
  • LY5
  • Lyt-4
  • PTPRC
  • RT7
  • si:dkeyp-47c5.1
  • T200
Reactivity
Cat (Feline), Human, Mouse (Murine)
1212
715
216
143
62
51
41
38
24
21
18
8
5
5
4
3
3
2
1
1
1
1
1
Host
Rat
1290
788
203
2
2
Clonality (Clone)
Monoclonal ()
Conjugate
This CD45 antibody is un-conjugated
281
227
168
103
48
48
47
41
40
29
27
27
26
26
25
24
24
24
24
20
17
17
16
16
14
14
10
9
9
7
6
6
6
5
5
5
5
5
5
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
1822
509
495
459
428
374
322
217
189
48
46
40
26
12
11
10
7
7
6
6
4
4
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Supplier
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Available images

Immunogen Mouse pre-B tumour cells (RAW112). Spleen cells from immunised Lewis rats were fused with cells of the rat S194/5 XX0.BU-1 myeloma cell line.
Clone RA3-6B2
Isotype IgG2a
Specificity CD45R
Purification Affinity Chromatography on Protein G.
Alternative Name CD45 / LCA (PTPRC Antibody Abstract)
Background CD45R is a member of the protein tyrosine phosphatase (PTP) family and a major cell surface glycoprotein. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. CD45R represents a restricted form of the CD45 family which primarily recognizes only cells of B lineage from proB cell through mature B lymphocytes and, prior to the availability of anti CD19 MAbs, was commonly used as a pan B cell marker. It also reacts with certain activated T cells, as well as non MHC restricted lytically active lymphokine activated killer (LAK) cells. CD45R contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. It is specifically expressed in hematopoietic cells and has been shown to be an essential regulator of T and B cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. CD45R also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported.Synonyms: L-CA, Leukocyte common antigen, PTPRC, Receptor-type tyrosine-protein phosphatase C, T200
Gene ID 10090
UniProt P06800
Application Notes Flow Cytometry: Use 10 μL of 1/100-1/200 diluted antibody to label 10^6 cells in 100 μL. Immunoprecipitation. Immunohistochemistry on Frozen and Paraffin Sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
 image for anti-CD45 antibody (Protein tyrosine Phosphatase, Receptor Type, C) (ABIN118214) Staining of mouse spleen cells with RAT ANTI MOUSE CD45R (ABIN118214).
Background publications Bertilaccio, Simonetti, Dagklis, Rocchi, Rodriguez, Apollonio, Mantovani, Ponzoni, Ghia, Garlanda, Caligaris-Cappio, Muzio: "Lack of TIR8/SIGIRR triggers progression of chronic lymphocytic leukemia in mouse models." in: Blood, Vol. 118, Issue 3, pp. 660-9, 2011 (PubMed).

Iskandar, Griffeth, Sharma: "Marijuana-induced mania in a healthy adolescent: a case report." in: General hospital psychiatry, Vol. 33, Issue 6, pp. 640.e3-4, 2011 (PubMed).

Lundqvist, Larsson, Nelson, Andersson, Bergström, Persson: "Concomitant infection decreases the malaria burden but escalates relapsing fever borreliosis." in: Infection and immunity, Vol. 78, Issue 5, pp. 1924-30, 2010 (PubMed).

Kleiter, Song, Lukas, Hasan, Neumann, Croxford, Pedré, Hövelmeyer, Yogev, Mildner, Prinz, Wiese, Reifenberg, Bittner, Wiendl, Steinman, Becker, Bogdahn, Neurath, Steinbrecher, Waisman: "Smad7 in T cells drives T helper 1 responses in multiple sclerosis and experimental autoimmune encephalomyelitis." in: Brain : a journal of neurology, Vol. 133, Issue Pt 4, pp. 1067-81, 2010 (PubMed).

Lacroix-Lamandé, Rochereau, Mancassola, Barrier, Clauzon, Laurent: "Neonate intestinal immune response to CpG oligodeoxynucleotide stimulation." in: PLoS ONE, Vol. 4, Issue 12, pp. e8291, 2009 (PubMed).

Herrmann, Kellert, Schmidt, Mildner, Hanisch, Brück, Prinz, Nau: "Streptococcus pneumoniae Infection aggravates experimental autoimmune encephalomyelitis via Toll-like receptor 2." in: Infection and immunity, Vol. 74, Issue 8, pp. 4841-8, 2006 (PubMed).

Rosmalen, Martin, Dobbs, Voerman, Drexhage, Haskins, Leenen: "Subsets of macrophages and dendritic cells in nonobese diabetic mouse pancreatic inflammatory infiltrates: correlation with the development of diabetes." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 80, Issue 1, pp. 23-30, 2000 (PubMed).

Monteith, Chelack, Davis, Haines: "Identification of monoclonal antibodies for immunohistochemical staining of feline B lymphocytes in frozen and formalin-fixed paraffin-embedded tissues." in: Canadian journal of veterinary research = Revue canadienne de recherche vétérinaire, Vol. 60, Issue 3, pp. 193-8, 1996 (PubMed).

Whiteland, Nicholls, Shimeld, Easty, Williams, Hill: "Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 43, Issue 3, pp. 313-20, 1995 (PubMed).

Ballas, Rasmussen: "Lymphokine-activated killer (LAK) cells. IV. Characterization of murine LAK effector subpopulations." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 144, Issue 1, pp. 386-95, 1990 (PubMed).

Asensi, Kimeno, Kawamura, Sakumoto, Nomoto: "Treatment of autoimmune MRL/lpr mice with anti-B220 monoclonal antibody reduces the level of anti-DNA antibodies and lymphadenopathies." in: Immunology, Vol. 68, Issue 2, pp. 204-8, 1989 (PubMed).

Spangrude, Heimfeld, Weissman: "Purification and characterization of mouse hematopoietic stem cells." in: Science (New York, N.Y.), Vol. 241, Issue 4861, pp. 58-62, 1988 (PubMed).

Spangrude, Muller-Sieburg, Heimfeld, Weissman: "Two rare populations of mouse Thy-1lo bone marrow cells repopulate the thymus." in: The Journal of experimental medicine, Vol. 167, Issue 5, pp. 1671-83, 1988 (PubMed).

Holmes, Langdon, Fredrickson, Coffman, Hoffman, Hartley, Morse: "Analysis of neoplasms induced by Cas-Br-M MuLV tumor extracts." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 137, Issue 2, pp. 679-88, 1986 (PubMed).

Coffman: "Surface antigen expression and immunoglobulin gene rearrangement during mouse pre-B cell development." in: Immunological reviews, Vol. 69, pp. 5-23, 1983 (PubMed).

HIRASHIMA, TAKAKU: "Experimental studies on erythropoietin. II. The relationship between juxtaglomerular cells and erythropoietin." in: Blood, Vol. 20, pp. 1-8, 1970 (PubMed).