Integrin, alpha 6 (ITGA6) antibody (Biotin)

Antigen: Integrin, alpha 6 (ITGA6)

Synonyms: CD49f, VLA-6, ITGA6B, FLJ18737, DKFZp686J01244, inta6, ITGA6, im:6897425, si:dkey-32o22.1, LOC100221694

Clonality: Monoclonal (NKI-GoH3)

Host: Rat

Reactivity: Dog (Canine)

Conjugate: Biotin

Application: Flow Cytometry (FACS)

Catalog no.: ABIN118231

Additional Information

Alternative name: CD49f / ITGA6

UniProt: P23229

Immunogen: BALB/c mouse mammary tumour cells. Spleen cells from immunised Sprague-Dawley rats were fused with cells of the SP2/0 mouse myeloma cell line

Cross-Reactivity: Dog (Canine), Human, Monkey, Mouse (Murine), Pig (Porcine)

Isotype: IgG2a

Clone: NKI-GoH3

Description: CD49F is a 120kD cell surface glycoprotein that can form distinct complexes with CD29 (VLA beta-chain), resulting in the VLA-6 (alpha-6 beta-1) complex, which is expressed on human platelets, or with the beta-4 integrin resulting in the alpha-6 beta-4 complex which is expressed on various human epithelial cells. Alternate Names: ITGA6, VLA-6, VLA6, Integrin alpha-6, Laminin Receptor, CD49 antigen-like family member F.

Specificity: This antibody recognises CD49F (VLA-6 alpha chain). Clone NKI-GoH3 reacts with platelets, megakaryocytes, T lymphocytes and common Acute Lymphoblastic Leukaemia cells (alpha-6 beta-1). In immunohistology the monoclonal antibody reacts with epithelial cells of a variety of tissues, peripheral nerves, microvascular endothelial cells, placenta cyto- and syncytotrophoblasts.VLA-6 is an important mediator of cell binding to laminin. Studies suggest that clone GoH3 specifically blocks the binding of cells to the E8 fragment of laminin.

Application Details

Application Notes: Flow cytometry: use 10 ul of neat - 1/5 diluted antibody to label 10e6 cells in 100ul. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.1 mg/ml

Buffer: PBS, pH7.4 containing 0.09% Sodium Azide and 1% Bovine Serum Albumin

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Restrictions: For Research Use only

Images

Staining of human peripheral blood lymphocytes with RAT ANTI HUMAN/MOUSE CD49f:BIOTIN

Publications

Sonnenberg, Linders, Modderman et al.: "Integrin recognition of different cell-binding fragments of laminin (P1, E3, E8) and evidence that alpha 6 beta 1 but not alpha 6 beta 4 functions as a major receptor for fragment E8." in: The Journal of cell biology, Vol. 110, Issue 6, pp. 2145-55, 1990 (PubMed).

Sonnenberg, Daams, Van der Valk et al.: "Development of mouse mammary gland: identification of stages in differentiation of luminal and myoepithelial cells using monoclonal antibodies and polyvalent antiserum against keratin." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 34, Issue 8, pp. 1037-46, 1986 (PubMed).

Sonnenberg, Modderman, Hogervorst: "Laminin receptor on platelets is the integrin VLA-6." in: Nature, Vol. 336, Issue 6198, pp. 487-9, 1989 (PubMed).

Sonnenberg, Janssen, Hogervorst et al.: "A complex of platelet glycoproteins Ic and IIa identified by a rat monoclonal antibody." in: The Journal of biological chemistry, Vol. 262, Issue 21, pp. 10376-83, 1987 (PubMed).

Galkowska, Waldemar, Wojewodzka: "Reactivity of antibodies directed against human antigens with surface markers on canine leukocytes." in: Veterinary immunology and immunopathology, Vol. 53, Issue 3-4, pp. 329-34, 1997 (PubMed).