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ITGA6 antibody (Integrin, alpha 6) (Biotin)

Details for Product anti-ITGA6 Antibody No. ABIN118231, Supplier: Log in to see
Antigen
  • 5033401O05Rik
  • AI115430
  • CD49f
  • Cd49f
  • im:6897425
  • inta6
  • itga6
  • ITGA6
  • ITGA6B
  • LOC100221694
  • si:dkey-32o22.1
  • VLA-6
  • wu:fb80g02
Reactivity
Dog (Canine), Human, Monkey, Mouse (Murine), Pig (Porcine)
323
118
38
25
17
16
11
8
6
2
2
2
2
1
1
Host
Rat
123
118
108
2
Clonality (Clone)
Monoclonal ()
Conjugate
This ITGA6 antibody is conjugated to Biotin
39
33
21
9
6
5
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
223
105
92
80
58
50
30
21
11
10
7
5
4
2
2
2
2
1
Supplier
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Immunogen BALB/c mouse mammary tumour cells. Spleen cells from immunised Sprague-Dawley rats were fused with cells of the SP2/0 mouse myeloma cell line
Clone NKI-GoH3
Isotype IgG2a
Specificity This antibody recognises CD49F (VLA-6 alpha chain). Clone NKI-GoH3 reacts with platelets, megakaryocytes, T lymphocytes and common Acute Lymphoblastic Leukaemia cells (alpha-6 beta-1). In immunohistology the monoclonal antibody reacts with epithelial cells of a variety of tissues, peripheral nerves, microvascular endothelial cells, placenta cyto- and syncytotrophoblasts.VLA-6 is an important mediator of cell binding to laminin. Studies suggest that clone GoH3 specifically blocks the binding of cells to the E8 fragment of laminin.
Purification Affinity chromatography on Protein G
Alternative Name CD49f / ITGA6 (ITGA6 Antibody Abstract)
Background CD49F is a 120kD cell surface glycoprotein that can form distinct complexes with CD29 (VLA beta-chain), resulting in the VLA-6 (alpha-6 beta-1) complex, which is expressed on human platelets, or with the beta-4 integrin resulting in the alpha-6 beta-4 complex which is expressed on various human epithelial cells.Synonyms: CD49 antigen-like family member F, Integrin alpha-6, VLA-6, VLA6
Gene ID 3655
NCBI Accession NP_000201
UniProt P23229
Application Notes Flow cytometry: use 10 μL of neat - 1/5 diluted antibody to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.1 mg/mL
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Flow Cytometry (FACS) image for anti-ITGA6 antibody (Integrin, alpha 6)  (Biotin) (ABIN118231) Staining of human peripheral blood lymphocytes with RAT ANTI HUMAN/MOUSE CD49f:BIOTIN
Background publications Galkowska, Waldemar, Wojewodzka: "Reactivity of antibodies directed against human antigens with surface markers on canine leukocytes." in: Veterinary immunology and immunopathology, Vol. 53, Issue 3-4, pp. 329-34, 1997 (PubMed).

Sonnenberg, Linders, Modderman, Damsky, Aumailley, Timpl: "Integrin recognition of different cell-binding fragments of laminin (P1, E3, E8) and evidence that alpha 6 beta 1 but not alpha 6 beta 4 functions as a major receptor for fragment E8." in: The Journal of cell biology, Vol. 110, Issue 6, pp. 2145-55, 1990 (PubMed).

Sonnenberg, Modderman, Hogervorst: "Laminin receptor on platelets is the integrin VLA-6." in: Nature, Vol. 336, Issue 6198, pp. 487-9, 1989 (PubMed).

Hemler, Crouse, Takada, Sonnenberg: "Multiple very late antigen (VLA) heterodimers on platelets. Evidence for distinct VLA-2, VLA-5 (fibronectin receptor), and VLA-6 structures." in: The Journal of biological chemistry, Vol. 263, Issue 16, pp. 7660-5, 1988 (PubMed).

Sonnenberg, Janssen, Hogervorst, Calafat, Hilgers: "A complex of platelet glycoproteins Ic and IIa identified by a rat monoclonal antibody." in: The Journal of biological chemistry, Vol. 262, Issue 21, pp. 10376-83, 1987 (PubMed).

Sonnenberg, Daams, Van der Valk, Hilkens, Hilgers: "Development of mouse mammary gland: identification of stages in differentiation of luminal and myoepithelial cells using monoclonal antibodies and polyvalent antiserum against keratin." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 34, Issue 8, pp. 1037-46, 1986 (PubMed).