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CD69 antibody (CD69) (Biotin)

Details for Product anti-CD69 Antibody No. ABIN118236, Supplier: Login to see New
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Synonyms 5830438K24Rik, AI452015, AIM, VEA, BL-AC/P26, CLEC2C, EA1, GP32/28, MLR-3
Mouse (Murine)
(214), (150), (28), (5), (5), (5), (5), (5)
(147), (104), (74), (20)
Clonality (Clone)
Monoclonal ()
This CD69 antibody is conjugated to Biotin
(50), (40), (21), (18), (11), (9), (6), (5), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Functional Studies (Func), Flow Cytometry (FACS), Immunoprecipitation (IP)
(261), (85), (54), (43), (40), (25), (21), (19), (12), (10), (9), (5), (4), (3), (2), (2), (2), (2), (2), (1)
Pubmed 9 references available
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Quantity 0.1 mg
Shipping to United States ( )
Availability Will be delivered in 6 to 8 Business Days
Clone HI-2F3
Isotype IgG
Specificity This antibody reacts with mouse CD69, which is inducibly expressed on T cells, B cells, NK cells, monocytes, and neutrophils (1,3). It induces T cell proliferation in the presence of PMA and FcR-bearing accessory cells (1). Tissue Distribution by Flow Cytometry Analysis: (representative histogram) Mouse Strain: BALB/c Cell Concentration : 1x10e6 cells per test Antibody Concentration Used: 1.0 μg/10e6 cells Isotypic Control: Biotin Hamster IgG
Alternative Name CD69 (CD69 Antibody Abstract)
Background CD69 acts as a contimulatory molecule for T cell activation and proliferation and plays a role in the activation and differentiation of a wide variety of hematopoeitic cells (3).Synonyms: AIM, Activation inducer molecule, BL-AC/P26, C-type lectin domain family 2 member C, EA1, Early T-cell activation antigen p60, Early activation antigen CD69, GP32/28, Leu-23, MLR-3
Gene ID 12515
NCBI Accession NP_001028294
UniProt P37217
Research Area CD Antigens, Surface Receptors of Immune Cells, Immunology
Application Notes Flow cytometry (for details please see specificity / protocols). Immunoprecipitation. In vitro T cell and NK cell activation (1,7).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.1 mg/mL
Buffer PBS, 0.09 % NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. Dilute only prior to immediate use
Storage 4 °C/-20 °C
Storage Comment Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Flow Cytometry (FACS) image for anti-CD69 antibody (CD69)  (Biotin) (ABIN118236) Hamster anti CD69 HI.2F3
Background publications Marzio, Jirillo, Ransijn et al.: "Expression and function of the early activation antigen CD69 in murine macrophages." in: Journal of leukocyte biology, Vol. 62, Issue 3, pp. 349-55, 1997 (PubMed).

Vilanova, Tavares, Ferreira et al.: "Role of monocytes in the up-regulation of the early activation marker CD69 on B and T murine lymphocytes induced by microbial mitogens." in: Scandinavian journal of immunology, Vol. 43, Issue 2, pp. 155-63, 1996 (PubMed).

Sobel, Yokoyama, Shevach et al.: "Aberrant expression of the very early activation antigen on MRL/Mp-lpr/lpr lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 150, Issue 2, pp. 673-82, 1993 (PubMed).

Ziegler, Levin, Johnson et al.: "The mouse CD69 gene. Structure, expression, and mapping to the NK gene complex." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 3, pp. 1228-36, 1994 (PubMed).

Brändle, Müller, Müller et al.: "Regulation of RAG-1 and CD69 expression in the thymus during positive and negative selection." in: European journal of immunology, Vol. 24, Issue 1, pp. 145-51, 1994 (PubMed).

Ziegler, Ramsdell, Alderson: "The activation antigen CD69." in: Stem cells (Dayton, Ohio), Vol. 12, Issue 5, pp. 456-65, 1995 (PubMed).

Yokoyama, Koning, Kehn et al.: "Characterization of a cell surface-expressed disulfide-linked dimer involved in murine T cell activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 141, Issue 2, pp. 369-76, 1988 (PubMed).

Yokoyama, Maxfield, Shevach: "Very early (VEA) and very late (VLA) activation antigens have distinct functions in T lymphocyte activation." in: Immunological reviews, Vol. 109, pp. 153-76, 1989 (PubMed).

Karlhofer, Yokoyama: "Stimulation of murine natural killer (NK) cells by a monoclonal antibody specific for the NK1.1 antigen. IL-2-activated NK cells possess additional specific stimulation pathways." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 146, Issue 10, pp. 3662-73, 1991 (PubMed).

Catalog No. ABIN118236
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