CD90 antibody (FITC)

Catalog No. ABIN118256

Product Details anti-CD90 Antibody (FITC)

Target: CD90 (THY1)

Reactivity: Mouse, Rat, Guinea Pig, Rabbit

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD90 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Specificity: This antibody recognises CD90, also known as Thy1.1. Since Thy1.1 is a monomorphic determinant in Rat but polymorphic in mice, clone MRC OX-7 reacts with Thy1.1 mice e.g. AKR and FVB mice, but not Thy1.2 mice such as CBA and BALB/c. Clone MRC OX-7 has been demonstrated to promote neurite outgrowths on peripherin-stained sympathetic neurons, using Fluorescence Microscopy (See Jeng, C .J. et al. for details). The Affinity of the Fab' of MRC OX-7 for Rat Thy1 is 3 x 109m-1 and Mouse Thy1.1 is 3 x 108m-1 (See Mason, D.W. & Williams, A.F. for details). This clone has been reported to induce glomerular nephritis in Wistar rats (See Tamura, M. et al. for details).

Purification: Affinity Chromatography on Protein G

Immunogen: Rat Thy1 antigen. Spleen cells of immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.

Clone: OX-7

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: Use 10 μL of Neat-1/10 diluted antibody to label 10^6 cells in 100 μL. This product is routinely tested on rat thymocytes.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, 0.09 % Sodium Azide, 1 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD90

Target: CD90 (THY1)

Alternative Name: CD90 (THY1 Products)

Synonyms: CD90 antibody, CD7 antibody, T25 antibody, Thy-1 antibody, Thy-1.2 antibody, Thy1.1 antibody, Thy1.2 antibody, Thy-1 cell surface antigen antibody, thymus cell antigen 1, theta antibody, THY1 antibody, Thy1 antibody

Background: Thy1.1 is expressed on a variety of cell types including thymocytes, neuronal cells, stem cells, T lymphocytes (mouse), immature B cells (rat) and connective tissues.Synonyms: CDw90, THY1, Thy-1, Thy-1 membrane glycoprotein

Gene ID: 21838

NCBI Accession: NP_033408

UniProt: P01831

Pathways: Cell-Cell Junction Organization