CD90 antibody

Catalog No. ABIN118259

Product Details anti-CD90 Antibody

Target: CD90 (THY1)

Reactivity: Mouse, Rat, Guinea Pig, Rabbit

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD90 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody recognises Rat and Mouse CD90, also known as Thy1.1, expressed on a variety of cell types including thymocytes, neuronal cells, stem cells, T lymphocytes (Mouse), immature B cells (rat) and connective tissues. Since Thy1.1 is a monomorphic determinant in Rat but polymorphic in Mice, clone MRC OX-7 reacts with Thy1.1 Mice e.g. AKR and FVB Mice, but not Thy1.2 Mice such as CBA and BALB/c. Clone MRC OX-7 has been demonstrated to promote neurite outgrowths on peripherin-stained sympathetic neurons, using fluorescence microscopy. (5) This clone has been reported to induce glomerular nephritis in Wistar Rats. (8) Affinity of the Fab' of MRC OX-7: Rat Thy1: 3 x 10e9me1 Mouse Thy1.1: 3 x 10e8m-1 (1).

Purification: Affinity Chromatography on Protein G.

Immunogen: Rat Thy 1 antigen. Remarks: Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.

Clone: OX-7

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: 1/50 - 1/100, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on frozen sections. Immunoprecipitation. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.2 containing 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-CD90 antibody (ABIN118259)

Shi, Liang, Guo, Zhang, Hui, Chang, Tao: "Adipose-Derived Stem Cells Cocultured with Chondrocytes Promote the Proliferation of Chondrocytes." in: Stem cells international, Vol. 2017, pp. 1709582, (2017) (PubMed).

Target Details for CD90

Target: CD90 (THY1)

Alternative Name: CD90 (THY1 Products)

Synonyms: CD90 antibody, CD7 antibody, T25 antibody, Thy-1 antibody, Thy-1.2 antibody, Thy1.1 antibody, Thy1.2 antibody, Thy-1 cell surface antigen antibody, thymus cell antigen 1, theta antibody, THY1 antibody, Thy1 antibody

Background: Thy1.1 is expressed on a variety of cell types including thymocytes, neuronal cells, stem cells, T lymphocytes (mouse), immature B cells (rat) and connective tissues.Synonyms: CDw90, THY1, Thy-1, Thy-1 membrane glycoprotein

Gene ID: 21838

NCBI Accession: NP_033408

UniProt: P01831

Pathways: Cell-Cell Junction Organization