T Cell Receptor (TCR) Vb 8.1, 8.2 antibody (PE,Cy5)

Antigen: T Cell Receptor (TCR) Vb 8.1, 8.2

Clonality: Monoclonal (KJ16)

Host: Rat

Reactivity: Mouse (Murine)

Conjugate: PE,Cy5

Application: Flow Cytometry (FACS)

Catalog no.: ABIN118345

Additional Information

Isotype: IgG1

Clone: KJ16

Specificity: This antibody reacts with mouse V beta 8.1, 8.2 T cell Receptor antigen. This antibody is provided in liquid format for application in single, dual, or three colour flow cytometry. 1 mg is the maximum amount of antibody required for 1 x 10e6 cells. Lesser amounts of antibody may be sufficient and it is recommended that each investigator determine dilutions appropriate for individual use. Appropriate controls should always be included in any labelling study.

Application Details

Application Notes: Flow Cytometry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: PE-Cy5 conjugates require a 650 nm long pass filter in the FL3 channel. FL2-FL3 compensation will be in the range of 1%.

Concentration: 0.2 mg/ml

Buffer: PBS, 0.09% NaN3 and a highly purified grade of BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml.

Storage: Store the antibody undiluted at 2-8°C. DO NOT FREEZE! This product is photosensitive and should protected from light. Shelf Life: one year from despatch.

Research Area: Immunology, Receptors

Restrictions: For Research Use only

Images

Tissue Distribution by Flow Cytometry Analysis: Mouse strain: BALB/c Cell Concentration: 1 x 106 cells per test Antibody Concentration Used: 1.0 mg/106 cells Isotypic Control: PE-Cy-5 Rat IgG1 (CLCR100) LFL 3 Cell Source: Thymus Percentage of cells stained above control: 11.5%

Publications

Publications: Sim, Augustin: "V beta gene polymorphism and a major polyclonal T cell receptor idiotype." in: Cell, Vol. 42, Issue 1, pp. 89-92, 1985 (PubMed).

Ignatowicz, Kappler, Marrack et al.: "Identification of two V beta 7-specific viral superantigens." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 1, pp. 65-71, 1994 (PubMed).