Mouse anti-Human Immunoglobulin J Polypeptide, Linker Protein For Immunoglobulin alpha and mu Polypeptides (IGJ) (Joining Chain) Antibody

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Antigen
Epitope
Joining Chain
5
3
2
2
1
1
1
Reactivity
Human
15
3
3
Host
Mouse
11
3
1
Clonality (Clone)
Monoclonal ()
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
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Immunogen Human J Chain. Remarks: Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Clone MC19-9
Isotype IgG1
Specificity The antibody reacts with Human J chain as tested by ELISA using purified Human Ig fragments as the solid phase target antigen. The antibody does not detect J chain in intact IgM in ELISA assays due to steric hindrance. In Western blotting techniques SM1013P detects J chain in IgM only in reducing conditions.
Purification Affinity Chromatography on Protein A.
Alternative Name Immunoglobulin J Chain
Background Immunoglobulin J chain (IGJ) serves to link two monomer units of either IgM or IgA. In the case of IgM, the J chain joined dimer is a nucleating unit for the IgM pentamer, and in the case of IgA it induces larger polymers. It also helps to bind these immunoglobulins to secretory components.Synonyms: IGCJ, IGJ
Gene ID 3512
NCBI Accession NP_653247
UniProt P01591
Application Notes Immunohistochemistry on Frozen Sections (1/200). ELISA (20 μg/mL). Immunoprecipitation (20 μg/mL). Western blotting (only under Reducing Conditions).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer TRIS buffered Saline containing 0.09 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.
Background publications Arnold, Wormald, Suter, Radcliffe, Harvey, Dwek, Rudd, Sim: "Human serum IgM glycosylation: identification of glycoforms that can bind to mannan-binding lectin." in: The Journal of biological chemistry, Vol. 280, Issue 32, pp. 29080-7, 2005 (PubMed).

Product cited in: Leithäuser, Bäuerle, Huynh, Möller: "Isotype-switched immunoglobulin genes with a high load of somatic hypermutation and lack of ongoing mutational activity are prevalent in mediastinal B-cell lymphoma." in: Blood, Vol. 98, Issue 9, pp. 2762-70, 2001 (PubMed).