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Mouse anti-Rat (Rattus) IgG1 (Heavy Chain) Antibody

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Heavy Chain
(99), (55), (17), (15), (12), (11), (6), (6), (5), (4), (4), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Rat (Rattus)
(255), (115), (91), (30), (18), (8), (6), (3), (3), (2), (2), (2), (1), (1), (1)
(170), (158), (93), (80), (28), (3), (1)
Clonality (Clone)
Monoclonal ()
Enzyme Immunoassay (EIA)
Pubmed 2 references available
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Quantity 0.25 mg
Shipping to United States ( )
Availability Will be delivered in 6 to 8 Business Days
Immunogen Rat IR27 IgG1 myeloma. Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Clone MARG1-2
Isotype IgG1
Purification Affinity Chromatography
Background Synonyms: Rat Immunoglobulin G1
Research Area Secondary Antibodies
Application Notes ELISA: 3 μg/mL - 3 μg/mL, This antibody may be used as a coating antibody in ELISA in combination with clone MARK-1/MARL-15 as detection reagent and purified rat IgG1 (SA013).

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4, 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.
Background publications Pelegrí, Castell, Serra et al.: "Prevention of adjuvant arthritis by the W3/25 anti-CD4 monoclonal antibody is associated with a decrease of blood CD4(+)CD45RC(high) T cells." in: Clinical and experimental immunology, Vol. 125, Issue 3, pp. 470-7, 2001 (PubMed).

Sato, Balla, Otterbein et al.: "Carbon monoxide generated by heme oxygenase-1 suppresses the rejection of mouse-to-rat cardiac transplants." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 6, pp. 4185-94, 2001 (PubMed).

Catalog No. ABIN118436
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