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CD3 antibody (CD3)

Details for Product anti-CD3 Antibody No. ABIN118479, Supplier: Log in to see
Antigen
  • CD3
Reactivity
Human, Primate
776
265
83
42
32
29
26
17
13
12
10
9
7
7
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
2
1
Host
Mouse
864
181
62
47
22
Clonality (Clone)
Monoclonal ()
Conjugate
This CD3 antibody is un-conjugated
157
115
94
65
27
23
22
21
21
20
17
17
14
12
11
10
10
8
7
6
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6
4
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4
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3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
937
251
218
134
130
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110
92
75
61
19
18
16
12
11
11
10
10
7
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
1
1
1
Supplier
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Immunogen Human infant thymocytes and lymphocytes from a patient with Sezary Syndrome. Remarks: Spleen cells from immunised BALB/c mice were fused with cells of the P3/NS1/1-Ag4-1 mouse myeloma cell line.
Clone UCHT1
Isotype IgG1
Specificity This antibody recognises a surface protein of peripheral blood T cells of molecular weight 19 kDa. As a pan T cell marker this antibody is present on more than 95 % of circulating Human peripheral T cells.
Purification Affinity Chromatography on Protein G
Endotoxin Level Low Endotoxin
Alternative Name CD3 (CD3 Antibody Abstract)
Background T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82 % of normal peripheral blood lymphocytes, 65-85 % of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.Synonyms: T-cell surface antigen T3/Leu-4, T-cell surface glycoprotein CD3, T3/Leu-4
Gene ID 916
NCBI Accession NP_000724
UniProt P07766
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, Hematopoietic Stem Cells, CD Antigens, Surface Receptors of Immune Cells
Application Notes Flow Cytometry: 1/50 - 1/100, Use 10 μL of the suggested working dilution to label 100 μL of whole blood. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: Endotoxin Level: less than 0.01 EU/ug
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS without preservatives
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
Background publications Zarkesh-Esfahani, Pockley, Metcalfe, Bidlingmaier, Wu, Ajami, Weetman, Strasburger, Ross: "High-dose leptin activates human leukocytes via receptor expression on monocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 8, pp. 4593-9, 2001 (PubMed).

Maggiorella, Monardo, Koanga-Mogtomo, Cioè, Sernicola, Corrias, Baroni, Verani, Titti: "Detection of infectious simian immunodeficiency virus in B- and T-cell lymphomas of experimentally infected macaques." in: Blood, Vol. 91, Issue 9, pp. 3103-11, 1998 (PubMed).

Clark, Ledbetter: "Leukocyte cell surface enzymology: CD45 (LCA, T200) is a protein tyrosine phosphatase." in: Immunology today, Vol. 10, Issue 7, pp. 225-8, 1989 (PubMed).

Clevers, Alarcon, Wileman, Terhorst: "The T cell receptor/CD3 complex: a dynamic protein ensemble." in: Annual review of immunology, Vol. 6, pp. 629-62, 1988 (PubMed).

Clevers, Dunlap, Terhorst: "The transmembrane orientation of the epsilon chain of the TcR/CD3 complex." in: European journal of immunology, Vol. 18, Issue 5, pp. 705-10, 1988 (PubMed).

Campana, Thompson, Amlot, Brown, Janossy: "The cytoplasmic expression of CD3 antigens in normal and malignant cells of the T lymphoid lineage." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 138, Issue 2, pp. 648-55, 1987 (PubMed).

Erber, Mynheer, Mason: "APAAP labelling of blood and bone-marrow samples for phenotyping leukaemia." in: Lancet, Vol. 1, Issue 8484, pp. 761-5, 1986 (PubMed).

Grogan, Fielder, Rangel, Jolley, Wirt, Hicks, Miller, Brooks, Greenberg, Jones: "Peripheral T-cell lymphoma: aggressive disease with heterogeneous immunotypes." in: American journal of clinical pathology, Vol. 83, Issue 3, pp. 279-88, 1985 (PubMed).

Erber, Pinching, Mason: "Immunocytochemical detection of T and B cell populations in routine blood smears." in: Lancet, Vol. 1, Issue 8385, pp. 1042-6, 1984 (PubMed).

Clark, Martin, Hansen, Ledbetter: "Evolution of epitopes on human and nonhuman primate lymphocyte cell surface antigens." in: Immunogenetics, Vol. 18, Issue 6, pp. 599-615, 1984 (PubMed).

Meuer, Acuto, Hussey, Hodgdon, Fitzgerald, Schlossman, Reinherz: "Evidence for the T3-associated 90K heterodimer as the T-cell antigen receptor." in: Nature, Vol. 303, Issue 5920, pp. 808-10, 1983 (PubMed).

Beverley, Callard: "Distinctive functional characteristics of human "T" lymphocytes defined by E rosetting or a monoclonal anti-T cell antibody." in: European journal of immunology, Vol. 11, Issue 4, pp. 329-34, 1981 (PubMed).

Kung, Goldstein, Reinherz, Schlossman: "Monoclonal antibodies defining distinctive human T cell surface antigens." in: Science (New York, N.Y.), Vol. 206, Issue 4416, pp. 347-9, 1979 (PubMed).