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CD4 antibody (PE)

CD4 Reactivity: Human FACS Host: Mouse Monoclonal RPA-T4 PE
Catalog No. ABIN118488
  • Target See all CD4 Antibodies
    CD4
    Reactivity
    • 355
    • 199
    • 113
    • 38
    • 24
    • 20
    • 19
    • 18
    • 15
    • 11
    • 10
    • 10
    • 8
    • 7
    • 6
    • 6
    • 6
    • 6
    • 6
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 328
    • 146
    • 108
    • 4
    • 2
    • 1
    • 1
    Mouse
    Clonality
    • 445
    • 138
    • 3
    Monoclonal
    Conjugate
    • 195
    • 65
    • 54
    • 46
    • 30
    • 12
    • 11
    • 10
    • 9
    • 9
    • 8
    • 8
    • 8
    • 7
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    This CD4 antibody is conjugated to PE
    Application
    • 470
    • 193
    • 99
    • 93
    • 81
    • 76
    • 71
    • 42
    • 39
    • 37
    • 33
    • 24
    • 10
    • 8
    • 7
    • 7
    • 6
    • 6
    • 6
    • 5
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS)
    Specificity
    This antibody recognises CD4. Epitope mapping studies have shown that antibodies, produced by clone RPA-T4, recognise an epitope within domain 1, of the extracellular region, of the CD4 Molecule. Clone RPA-T4 has been reported to block gp120-CD4 interaction and inhibit syncytium formation. We recommend the use of SM1058LE for this purpose.
    Purification
    Affinity Chromatography on Protein G
    Immunogen
    Human PHA blasts. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
    Clone
    RPA-T4
    Isotype
    IgG1
    Top Product
    Discover our top product CD4 Primary Antibody
  • Application Notes
    Flow Cytometry: Use 10 μL of neat-1/10 diluted antibody to label 10^6 cells or 100 μL of whole blood.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Reconstitution
    Restore with distilled water
    Concentration
    0.1 mg/mL
    Buffer
    PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Prior to and following reconstitution store the antibody at -20 °C.
  • Target
    CD4
    Alternative Name
    CD4 (CD4 Products)
    Synonyms
    cd4 antibody, L3T4 antibody, Ly-4 antibody, CD4mut antibody, W3/25 antibody, p55 antibody, CD4 molecule antibody, carcinoembryonic antigen-related cell adhesion molecule 5 antibody, T-cell surface glycoprotein CD4 antibody, CD4 antigen antibody, Cd4 molecule antibody, CD4 antibody, LOC100125525 antibody, cd4 antibody, Cd4 antibody
    Background
    CD4 is a 55kD cell surface glycoprotein that is primarily expressed on a subpopulation of T lymphocytes, on peripheral blood monocytes and on tissue macrophages.Synonyms: T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
    Gene ID
    920
    NCBI Accession
    NP_000607
    UniProt
    P01730
    Pathways
    TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events
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