CD14 antibody (FITC)

Catalog No. ABIN118506

Product Details anti-CD14 Antibody (FITC)

Target: CD14

Reactivity: Human, Monkey, Dog, Pig, Sheep, Rabbit, Cow, Goat, Cat, Mink, Llama

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD14 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Cross-Reactivity (Details): Species reactivity (expected):Canine, Goat, Cat, Rabbit, Mink, Bovine, Porcine, Sheep, Cynomolgus Monkey, Llama.
Species reactivity (tested):Human.

Purification: Affinity Chromatography on Protein G

Immunogen: CD14 Human

Clone: Tük4

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry: Use 10 μL of neat-1/10 diluted antibody to label 10^6 cells or 100 μL whole blood.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, 0.09 % Sodium Azide, 1 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-CD14 antibody (FITC) (ABIN118506)

Brückner, Tautenhahn, Winkler, Stock, Dollinger, Christ: "A fat option for the pig: hepatocytic differentiated mesenchymal stem cells for translational research." in: Experimental cell research, Vol. 321, Issue 2, pp. 267-75, (2014) (PubMed).

Brückner, Tautenhahn, Winkler, Stock, Jonas, Dollinger, Christ: "Isolation and hepatocyte differentiation of mesenchymal stem cells from porcine bone marrow--"surgical waste" as a novel MSC source." in: Transplantation proceedings, Vol. 45, Issue 5, pp. 2056-8, (2013) (PubMed).

Target Details for CD14

Target: CD14

Alternative Name: CD14 (CD14 Products)

Background: CD14 (also known lipopolysaccharide (LPS) receptor) is expressed strongly on monocytes and macrophage and weakly on the surface of neutrophils. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for complexes of LPS and LPS binding protein (LBP). Soluble CD14, also binding to LPS, acts at physiological concentration as an LPS agonist and has, at higher concentrations, an LPS antagonizing effect in cell activation.Synonyms: Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein

Gene ID: 9606

UniProt: P08571

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades