Interleukin 5 (IL5) antibody (PE)

Details for Product No. ABIN118521
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Antigen
Synonyms IL5, Il-5, IL-5, TRF, EDF, Pro-TRH
Reactivity
Guinea Pig, Human, Mouse (Murine)
(105), (78), (17), (12), (1)
Host
Rat
(77), (59), (6), (2), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
PE
(30), (4), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Flow Cytometry (FACS)
(82), (80), (37), (13), (13), (12), (11), (10), (10), (7), (7), (4), (3), (3), (3), (3), (2), (1), (1), (1), (1)
Pubmed 5 references available
Quantity 50 tests
Shipping to United States (Change)
Availability Will be delivered in 6 to 8 Business Days
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Catalog No. ABIN118521
511.50 $
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Immunogen Semi-purified natural murine IL-5.
Clone TRFK5
Isotype IgG1
Purification Affinity chromatography on Protein A
Alternative Name Interleukin-5 / IL5
Background Interleukin 5 (IL5) is a cytokine produced primarily by activated T lymphocytes. It exists as an antiparallel disulfide linked homodimeric glycoprotein with 115 amino acid residues in each chain. Known also as EDF (eosinophil differentiating factor), functions predominantly as an eosinophilopoietic factor. Analysis of its crystal structure reveals a novel two domain structure, with each domain showing significant homology to the cytokine fold in GMCSF, MCSF, IL2, IL4 and growth hormone. Human and mouse IL5 have 70 % amino acid sequence homology.Synonyms: B-cell differentiation factor I, Eosinophil differentiation factor, IL-5, T-cell replacing factor, TRF
Gene ID 3567
NCBI Accession NP_000870
UniProt P05113
Application Notes Flow Cytometry: Neat - 1:10, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL, Membrane permeabilisation is required for this application.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.1 mg/mL
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide and 0.5 % Bovine Serum Albumin
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Dilute only prior to immediate use
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C. DO NOT FREEZE! . Freezing Alkaline Phosphatase conjugates will result in a substantial loss of enzymatic activity.
Background publications Taguchi, McGhee, Coffman et al.: "Analysis of Th1 and Th2 cells in murine gut-associated tissues. Frequencies of CD4+ and CD8+ T cells that secrete IFN-gamma and IL-5." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 145, Issue 1, pp. 68-77, 1990 (PubMed).

Taguchi, McGhee, Coffman et al.: "Detection of individual mouse splenic T cells producing IFN-gamma and IL-5 using the enzyme-linked immunospot (ELISPOT) assay." in: Journal of immunological methods, Vol. 128, Issue 1, pp. 65-73, 1990 (PubMed).

Schumacher, OGarra, Shrader et al.: "The characterization of four monoclonal antibodies specific for mouse IL-5 and development of mouse and human IL-5 enzyme-linked immunosorbent." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 141, Issue 5, pp. 1576-81, 1988 (PubMed).

Sander, Höidén, Andersson et al.: "Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining." in: Journal of immunological methods, Vol. 166, Issue 2, pp. 201-14, 1994 (PubMed).

Portanova, Christine, Rangwala et al.: "Rapid and selective induction of blood eosinophilia in guinea pigs by recombinant human interleukin 5." in: Cytokine, Vol. 7, Issue 8, pp. 775-83, 1996 (PubMed).

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