CD33 antibody (CD33 Molecule) (APC)

Details for Product anti-CD33 Antibody No. ABIN118633, Supplier: Log in to see
Antigen
  • Siglec-3
  • gp67
  • SIGLEC-3
  • SIGLEC3
  • p67
  • CD33 molecule
  • CD33 antigen
  • CD33
  • Cd33
Reactivity
Human
600
39
31
24
16
8
4
2
1
Host
Mouse
484
138
1
1
Clonality (Clone)
Monoclonal ()
Conjugate
This CD33 antibody is conjugated to APC
63
54
36
26
16
14
14
14
12
11
9
9
9
9
9
8
8
5
4
4
4
4
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Flow Cytometry (FACS)
376
252
217
116
106
84
75
70
58
27
26
16
12
11
6
4
4
2
2
1
1
1
Options
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Supplier Product No.
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Immunogen Human AML cells. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NSI myeloma cell line.
Clone WM53
Isotype IgG1
Specificity This antibody recognises the CD33 cell surface glycoprotein.
Purification Affinity Chromatography on Protein G.
Alternative Name CD33 / SIGLEC3 (CD33 Antibody Abstract)
Background CD33 is found on granulocyte and macrophage precursors in the bone marrow, but is not on pluripotent stem cells. The protein is also expressed on, and is a useful marker for, peripheral monocytes. It is also useful for distinguishing myelogenous leukaemia cells from lymphoid or erythroid leukaemias. Identification of human Monocytes (bright) and Granulocytes (dim) expressing the 67 kDa M.W. surface antigen. CD33 is a 67kD molecule expressed by monocytes, granulocyte precursors, myeloid progenitor cells and myeloid leukaemias.Synonyms: Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, Siglec-3, gp67
Gene ID 945
NCBI Accession NP_001763
UniProt P20138
Research Area CD Antigens, Surface Receptors of Immune Cells
Application Notes Flow Cytometry: Use 10 μL of Neat-1/10 diluted antibody to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Reconstitution Restore with distilled water.
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice This product is photosensitive and should be protected from light
Storage 4 °C
Storage Comment Prior to and following reconstitution store the antibody at 2-8 °C. DO NOT FREEZE!
Supplier Images
 image for anti-CD33 Molecule (CD33) antibody (APC) (ABIN118633) Staining of human peripheral blood monocytes with MOUSE ANTI HUMAN CD33:APC (ABIN118633)
Background publications Favaloro, Bradstock, Kabral, Grimsley, Berndt: "Characterization of monoclonal antibodies to the human myeloid-differentiation antigen, 'gp67' (CD-33)." in: Disease markers, Vol. 5, Issue 4, pp. 215-25, 1988 (PubMed).

Favaloro, Bradstock, Kabral, Grimsley, Zowtyj, Zola et al.: "Further characterization of human myeloid antigens (gp160,95; gp150; gp67): investigation of epitopic heterogeneity and non-haemopoietic distribution using panels of monoclonal antibodies belonging ..." in: British journal of haematology, Vol. 69, Issue 2, pp. 163-71, 1988 (PubMed).