CD45 antibody

Catalog No. ABIN118681

Product Details anti-CD45 Antibody

Target: CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Reactivity: Human, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD45 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody recognises the low molecular weight isoform (180 kDa) of the Leucocyte Common Antigen (LCA).

Purification: Affinity Chromatography on Protein G.

Immunogen: Human IL-2 dependent T-Cells. Remarks: Spleen cells from immunised mice were fused with cells of the mouse P3/NS1/1-Ag4-1 myeloma cell line.

Clone: UCHL1

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry: Use 10 μL of 1/25-1/50 diluted antibody to label 10^6 cells in 100 μL. Immunoprecipitation. Immunohistochemistry on Frozen and Paraffin Sections: This product does not require protein digestion pre-treatment of paraffin sections, This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections. Recommended positive control tissue: Tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD45

Target: CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Alternative Name: CD45 / LCA (PTPRC Products)

Synonyms: PTPRC antibody, si:dkeyp-47c5.1 antibody, B220 antibody, CD45 antibody, CD45R antibody, GP180 antibody, L-CA antibody, LCA antibody, LY5 antibody, T200 antibody, Cd45 antibody, Ly-5 antibody, Lyt-4 antibody, loc antibody, Lca antibody, RT7 antibody, cd45 antibody, protein tyrosine phosphatase, receptor type C antibody, protein tyrosine phosphatase, receptor type, C antibody, receptor-type tyrosine-protein phosphatase C antibody, PTPRC antibody, ptprc antibody, LOC100074271 antibody, Ptprc antibody

Background: The antigen is expressed by a functional subset of T cells with memory phenotype. In peripheral blood UCHL1 stains 40-80 % of lymphocytes, and all monocytes and granulocytes.Synonyms: L-CA, Leukocyte common antigen, PTPRC, Receptor-type tyrosine-protein phosphatase C, T200

Gene ID: 5788

NCBI Accession: NP_002829

UniProt: P08575

Pathways: TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, BCR Signaling