Integrin beta 3 (ITGB3) antibody
| Antigen |
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| Synonyms |
LAD, CD18, MF17, MFI7, LCAMB, LFA-1, MAC-1, CD61, GP3A, GPIIIa, INGRB3, MGC159221, MGC159223, integrin, ITGB3 |
| Clonality |
Monoclonal (PM6-13) |
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Host
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Reactivity
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Human (238), Mouse (Murine) (127), Rat (Rattus) (70), Pig (Porcine) (22), Cow (Bovine) (20), Monkey (9), Primate (6), Dog (Canine) (2), Cat (Feline) (1), Chicken (1), Hamster (1), Horse (Equine) (1), Sheep (Ovine) (1)
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Conjugate
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FITC (43), PE (25), Biotin (17), RPE (7), Alexa Fluor 350 (3), Alexa Fluor 488 (3), Alexa Fluor 555 (3), Alexa Fluor 647 (3), Cy3 (3), Cy5 (3), Cy5.5 (3), Cy7 (3), Gold (3), HRP (3), PE,Cy3 (3), PE,Cy5 (3), PE,Cy5.5 (3), PE,Cy7 (3), APC (2)
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Application
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 Alternatives Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (IHC)
Flow Cytometry (FACS) (143), Western Blotting (WB) (100), Immunofluorescence (IF) (55), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (53), Immunohistochemistry (IHC) (38), ELISA (37), Immunoprecipitation (IP) (33), Immunohistochemistry (Frozen Sections) (IHC (fro)) (20), Immunohistochemistry (Fixed) (IHC (fx)) (17), Immunocytochemistry (ICC) (9), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (9), Functional Studies (Func) (4), Immunoelectron Microscopy (IEM) (3), Enzyme Immunoassay (EIA) (1)
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| Catalog no. |
ABIN118755 |
| Quantity |
1 mg (1 mg/ml) (Variants) |
| Price |
1,001.00 $ Plus shipping costs $45.00
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| Shipping to |
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| Availability |
Will be delivered in 6 to 8 Business Days |
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Alternative name
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CD61 / ITGB3
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Immunogen
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Platelet plasma membrane.
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Cross-Reactivity
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Human, Monkey
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Isotype
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IgG1
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Clone
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PM6-13
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Specificity
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This antibody recognises a 105kD glycoprotein, which is the integrin beta 3 chain. This molecule associates with either the alpha IIb integrin (CD41) or the alpha V integrin (CD51) at the cell surface. CD61 is expressed on platelets and megakaryocytes in association with CD41, and on endothelial cells, monocytes, platelets and osteoclasts in association with CD51. CD61 is alpha receptor for fibrinogen, fibronectin, vWF, vitronectin and thrombospondin. This antibody inhibits platelet aggregation and activation induced by thrombin and collagen. Cross reacts with baboon cells (3).
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Application Notes
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Flow cytometry: 1/10 – 1/50, use 10 ul of the suggested working dilution to label 106 cells. Immunohistochemistry on frozen sections: recommended positive control tissue: Bone marrow. Immunoprecipitation. Western Blot under non-reducing conditions. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
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Concentration
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1 mg/ml
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Purification
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Azide Free
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Buffer
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This antibody is supplied as liquid, Protein A affinity purified immunoglobulin fraction in PBS buffer pH 7.4 without preservatives.
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Storage
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Store at –20°C only. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
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Research Area
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CD Antigens, Surface Receptors of Immune Cells
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Restrictions
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For Research Use only
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Hynes: "Integrins: versatility, modulation, and signaling in cell adhesion." in: Cell, Vol. 69, Issue 1, pp. 11-25, 1992 (PubMed).
Michelson, Page, Casey et al.: "An eosinophilia-myalgia syndrome related disorder associated with exposure to L-5-hydroxytryptophan." in: The Journal of rheumatology, Vol. 21, Issue 12, pp. 2261-5, 1995 (PubMed).
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Alternatives for antigen "Integrin beta 3 (ITGB3)", type "Antibodies"
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Hosts
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Rabbit (128), Mouse (127), Hamster (34), Rat (1)
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Reactivities
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Human (238), Mouse (Murine) (127), Rat (Rattus) (70), Pig (Porcine) (22), Cow (Bovine) (20), Monkey (9), Primate (6), Dog (Canine) (2), Cat (Feline) (1), Chicken (1), Hamster (1), Horse (Equine) (1), Sheep (Ovine) (1)
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Applications
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Flow Cytometry (FACS) (143), Western Blotting (WB) (100), Immunofluorescence (IF) (55), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (53), Immunohistochemistry (IHC) (38), ELISA (37), Immunoprecipitation (IP) (33), Immunohistochemistry (Frozen Sections) (IHC (fro)) (20), Immunohistochemistry (Fixed) (IHC (fx)) (17), Immunocytochemistry (ICC) (9), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (9), Functional Studies (Func) (4), Immunoelectron Microscopy (IEM) (3), Enzyme Immunoassay (EIA) (1)
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Conjugates
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FITC (43), PE (25), Biotin (17), RPE (7), Alexa Fluor 350 (3), Alexa Fluor 488 (3), Alexa Fluor 555 (3), Alexa Fluor 647 (3), Cy3 (3), Cy5 (3), Cy5.5 (3), Cy7 (3), Gold (3), HRP (3), PE,Cy3 (3), PE,Cy5 (3), PE,Cy5.5 (3), PE,Cy7 (3), APC (2)
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Epitopes
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pTyr773 (31), pTyr785 (30), C-Term (22), N-Term (7), Tyr785 (4), Tyr773 (3), C-Term,AA 734-760 (1), C-Term,AA 740-769 (1), Internal Region (1)
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