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C5AR1 antibody (N-Term)

C5AR1 Reactivity: Human, Cow, Rabbit WB, FACS, IHC (p) Host: Mouse Monoclonal S5-1 unconjugated
Catalog No. ABIN118843
  • Target See all C5AR1 Antibodies
    C5AR1 (Complement Component 5a Receptor 1 (C5AR1))
    Binding Specificity
    • 16
    • 15
    • 9
    • 7
    • 7
    • 7
    • 7
    • 7
    • 6
    • 6
    • 5
    • 5
    • 5
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 1-31, N-Term
    Reactivity
    • 110
    • 19
    • 18
    • 17
    • 14
    • 10
    • 5
    • 5
    • 5
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Cow, Rabbit
    Host
    • 79
    • 29
    • 17
    • 1
    • 1
    Mouse
    Clonality
    • 79
    • 43
    • 2
    Monoclonal
    Conjugate
    • 60
    • 11
    • 11
    • 8
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This C5AR1 antibody is un-conjugated
    Application
    • 70
    • 61
    • 37
    • 18
    • 18
    • 14
    • 13
    • 13
    • 8
    • 8
    • 8
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Specificity
    This antibody recognises the C5a receptor (C5aR) CD88. Clone S5/1 was raised against a synthetic peptide comprising the N-terminal extracellular domain of the C5aR (met1-Asn31) and has recently been shown to recognise the heptameric peptide (D15DKDTLD21). Clone S5/1 has been shown to inhibit the binding of C5a to its receptor. We recommend the use of SM1182A for functional studies.
    Cross-Reactivity (Details)
    Species reactivity (expected):Bovine and Rabbit.
    Species reactivity (tested):Human
    Purification
    Ammonium sulphate precipitation
    Immunogen
    C5aR - peptide: Met1 - Asn31. Spleen cells from immunised BALB/c mice were fused with cells of the X63-Ag8 myeloma cell line.
    Clone
    S5-1
    Isotype
    IgG2a
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  • Application Notes
    Flow Cytometry: 1/50 - 1/100, Use 10 μL of the suggested working dilution to label 5 x 10e5 cells in 100 μL. Immunohistochemistry on paraffin sections. Western Blot. Functional Assays.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Buffer
    PBS containing 0.09 % Sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target
    C5AR1 (Complement Component 5a Receptor 1 (C5AR1))
    Alternative Name
    CD88 / C5R1 (C5AR1 Products)
    Synonyms
    C5A antibody, C5AR antibody, C5R1 antibody, CD88 antibody, C5aR antibody, C5r1 antibody, Cd88 antibody, D7Msu1 antibody, GPR77 antibody, complement C5a receptor 1 antibody, complement component 5a receptor 1 antibody, C5AR1 antibody, C5ar1 antibody, c5ar1 antibody
    Background
    CD88 is predominantly expressed on cells of the myeloid lineage.Synonyms: C5AR1, C5a anaphylatoxin chemotactic receptor, C5a-R, C5aR, Complement Component 5a Receptor 1
    Gene ID
    728
    NCBI Accession
    NP_001727
    UniProt
    P21730
    Pathways
    Complement System
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