Killer Cell Immunoglobulin-Like Receptor, Two Domains, Long Cytoplasmic Tail, 3 (KIR2DL3) antibody

Antigen: Killer Cell Immunoglobulin-Like Receptor, Two Domains, Long Cytoplasmic Tail, 3 (KIR2DL3)

Clonality: Monoclonal (GL183)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Functional Studies (Func), Immunoprecipitation (IP), Western Blotting (WB), Flow Cytometry (FACS)

Catalog no.: ABIN118907

Additional Information

Alternative name: CD158b / KIR2DL3

UniProt: P43628

Immunogen: NK cell clone E57 (CD2+, CD3-, CD7+, CD16+, CD56+)

Cross-Reactivity: Human

Isotype: IgG1

Clone: GL183

Application Details

Application Notes: Flow cytometry: 2 ug / 5 x 10e5 cells (or 100 ul whole blood), per test. Studies on the recognition mechanisms and function of NK cells and CTLs. Analysis of the NK cell repertoire. Immunoprecipitation of p50.2 and p58.2 molecules. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.2 mg/ml (prior to lyophilization)

Buffer: PBS containing 1 mg/ml BSA as stabilizer containing no preservative

Preservative: Azide Free

Storage: Store lyophilized product at 2-8 °C. After reconstitution, aliquot and store at -20 °C. Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: one year from despatch.

Research Area: CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Publications

Moretta, Tambussi, Bottino et al.: "A novel surface antigen expressed by a subset of human CD3- CD16+ natural killer cells. Role in cell activation and regulation of cytolytic function." in: The Journal of experimental medicine, Vol. 171, Issue 3, pp. 695-714, 1990 (PubMed).

Moretta, Bottino, Pende et al.: "Identification of four subsets of human CD3-CD16+ natural killer (NK) cells by the expression of clonally distributed functional surface molecules: correlation between subset assignment of NK clones and ability to mediate specific alloantigen recognition." in: The Journal of experimental medicine, Vol. 172, Issue 6, pp. 1589-98, 1991 (PubMed).

Moretta, Sivori, Vitale et al.: "Existence of both inhibitory (p58) and activatory (p50) receptors for HLA-C molecules in human natural killer cells." in: The Journal of experimental medicine, Vol. 182, Issue 3, pp. 875-84, 1995 (PubMed).

Wagtmann, Biassoni, Cantoni et al.: "Molecular clones of the p58 NK cell receptor reveal immunoglobulin-related molecules with diversity in both the extra- and intracellular domains." in: Immunity, Vol. 2, Issue 5, pp. 439-49, 1995 (PubMed).

Ferrini, Cambiaggi, Meazza et al.: "T cell clones expressing the natural killer cell-related p58 receptor molecule display heterogeneity in phenotypic properties and p58 function." in: European journal of immunology, Vol. 24, Issue 10, pp. 2294-8, 1994 (PubMed).

Biassoni, Cantoni, Falco et al.: "The human leukocyte antigen (HLA)-C-specific "activatory" or "inhibitory" natural killer cell receptors display highly homologous extracellular domains but differ in their transmembrane and intracytoplasmic portions." in: The Journal of experimental medicine, Vol. 183, Issue 2, pp. 645-50, 1996 (PubMed).

Mingari, Schiavetti, Ponte et al.: "Human CD8+ T lymphocyte subsets that express HLA class I-specific inhibitory receptors represent oligoclonally or monoclonally expanded cell populations." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 22, pp. 12433-8, 1996 (PubMed).

Lanier: "Natural killer cell receptors and MHC class I interactions." in: Current opinion in immunology, Vol. 9, Issue 1, pp. 126-31, 1997 (PubMed).