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Killer Cell Immunoglobulin-Like Receptor, Two Domains, Long Cytoplasmic Tail, 3 (KIR2DL3) antibody

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(51), (45)
Clonality (Clone)
Monoclonal ()
(9), (5), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Flow Cytometry (FACS), Functional Studies (Func), Immunoprecipitation (IP), Western Blotting (WB)
(44), (43), (24), (14), (11), (9), (7), (5), (3), (2), (2), (1), (1), (1)
Pubmed 8 references available
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Quantity 0.2 mg
Shipping to United States ( )
Immunogen NK cell clone E57 (CD2+, CD3-, CD7+, CD16+, CD56+)
Clone GL183
Isotype IgG1
Specificity GL183 monoclonal antibody immunoprecipitates the p58.2 Molecule, a 58 kDa type I transmembrane protein (1-3). It can also react with a lighter (p50.2, 50 kDa) molecule, highly homologous to p58.2 in the extracellular area, but exhibiting a shorter cytoplasmic tail.(4,5) These p58.2 and 50.2 Molecules are characterized by two immunoglobulin-like extracellular domains. They are expressed by human NK cell subsets and by human T lymphocytes subsets, the latest being, in most instances, CD8+CD4-TCR alpha beta+.(6,7) p58.2 and p50.2 are members of the newly described natural killer cell receptor (NK-R) family (for reviews, see 8,9). The GL183 antibody can restore in vitro the lysis by human NK clones of otherwise lysis-protected targets carrying the Cw3 or related alleles. GL183 antibody has been assigned to the CD158b cluster of differentiation of the VIth International Workshop on Human Leucocyte Differentiation Antigens (HLDA) in Kobe, 1996.
Alternative Name CD158b / KIR2DL3 (KIR2DL3 Antibody Abstract)
Background KIR2DL3 is a member of the killer cell immunoglobulin-like receptor (KIR) family. The KIR family consists of transmembrane glycoproteins of the Ig superfamily expressed on human NK cells and a subset of human T cells. They are involved in recognition of either MHC class I molecules or unknown ligand on target cells and inhibit cytotoxic activities.Synonyms: KIRCL23, Killer cell immunoglobulin-like receptor 2DL3, MHC class I NK cell receptor, NKAT2a, NKAT2b, Natural killer-associated transcript 2
Gene ID 3804
NCBI Accession NP_056952
UniProt P43628
Research Area CD Antigens, Surface Receptors of Immune Cells
Application Notes Flow cytometry: 2 μg / 5 x 10e5 cells (or 100 μL whole blood), per test. Studies on the recognition mechanisms and function of NK cells and CTLs. Analysis of the NK cell repertoire. Immunoprecipitation of p50.2 and p58.2 Molecules.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.2 mg/mL (prior to lyophilization)
Buffer PBS containing 1 mg/mL BSA as stabilizer containing no preservative
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C up to one week or at -20 °C for longer.
Background publications Lanier: "Natural killer cell receptors and MHC class I interactions." in: Current opinion in immunology, Vol. 9, Issue 1, pp. 126-31, 1997 (PubMed).

Mingari, Schiavetti, Ponte et al.: "Human CD8+ T lymphocyte subsets that express HLA class I-specific inhibitory receptors represent oligoclonally or monoclonally expanded cell populations." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 22, pp. 12433-8, 1996 (PubMed).

Biassoni, Cantoni, Falco et al.: "The human leukocyte antigen (HLA)-C-specific 'activatory' or 'inhibitory' natural killer cell receptors display highly homologous extracellular domains but differ in their transmembrane and ..." in: The Journal of experimental medicine, Vol. 183, Issue 2, pp. 645-50, 1996 (PubMed).

Ferrini, Cambiaggi, Meazza et al.: "T cell clones expressing the natural killer cell-related p58 receptor molecule display heterogeneity in phenotypic properties and p58 function." in: European journal of immunology, Vol. 24, Issue 10, pp. 2294-8, 1994 (PubMed).

Wagtmann, Biassoni, Cantoni et al.: "Molecular clones of the p58 NK cell receptor reveal immunoglobulin-related molecules with diversity in both the extra- and intracellular domains." in: Immunity, Vol. 2, Issue 5, pp. 439-49, 1995 (PubMed).

Moretta, Sivori, Vitale et al.: "Existence of both inhibitory (p58) and activatory (p50) receptors for HLA-C molecules in human natural killer cells." in: The Journal of experimental medicine, Vol. 182, Issue 3, pp. 875-84, 1995 (PubMed).

Moretta, Bottino, Pende et al.: "Identification of four subsets of human CD3-CD16+ natural killer (NK) cells by the expression of clonally distributed functional surface molecules: correlation between subset assignment of NK clones and ability to mediate specific alloantigen recognition." in: The Journal of experimental medicine, Vol. 172, Issue 6, pp. 1589-98, 1991 (PubMed).

Moretta, Tambussi, Bottino et al.: "A novel surface antigen expressed by a subset of human CD3- CD16+ natural killer cells. Role in cell activation and regulation of cytolytic function." in: The Journal of experimental medicine, Vol. 171, Issue 3, pp. 695-714, 1990 (PubMed).

Catalog No. ABIN118907

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