This antibody recognises the glucocorticoid receptor, located in the cytoplasm of cells, and associated with certain heat shock proteins. Clone 8E9 binds to an epitope between amino acids 167-176.
Purification
Affinity chromatography on Protein G
Immunogen
26 amino acid peptide corresponding to residues 150-176 of human GCR linked to thyroglobulin (human). Spleen cells from immunised BALB/c mice were fused with cells of the mouse Sp-2/0 Ag14 myeloma cell line.
NR3C1
Reactivity: Human
WB, IHC, ELISA
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: 1/1000. Western Blot: 1/1000. Successful use in Immunohistochemistry on frozen and paraffin sections was reported bysome researchers. As this detection method has not been verified by antibodies-online, theapplication was deleted from the database. This does not necessarily exclude the use insuch procedure. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS, pH 7.2 containing 0.09 % Sodium Azide and 0.1 % Bovine Serum Albumin
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Target
Glucocorticoid Receptor (NR3C1)
(Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) (NR3C1))
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Background
Steroid receptors are ligand-dependent, intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Glucocorticoids are a family of steroids necessary for the regulation of energy metabolism and the immune and inflammatory responses. These compounds exert their effect through their interaction with the glucocoticoid receptor (GR) and that complex's subsequent association with DNA. All normal mammalian tissues examined to date have been shown to contain glucocorticoid receptor.Synonyms: GR, GRL, NR3C1