alpha Defensin 1 antibody

Catalog No. ABIN118975

Product Details anti-alpha Defensin 1 Antibody

Target: alpha Defensin 1 (DEFA1) (Defensin, alpha 1 (DEFA1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This alpha Defensin 1 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Several Defensins are recognized. This antibody recognises a family of Cyclic Peptides in Neutrophils. Human: Defensin 1-3 (HNP-1 to HNP-3) in Human Neutrophils. Synthetic Defensin-1 and -2 (Bachem, Bubendorf CH) stain also positively. Not tested with Defensin-4. A side reaction to elastin has been observed in humans. Other: not tested. Defensins have been described in rabbits (NP-1 to NP-5, NP3a = Corticostatin), and in mice (cryptodin).

Purification: Affinity chromatography

Immunogen: Native defensins. The epitope has not been further characterized.

Clone: DEF-3

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry on Frozen Sections: 0.2 μg/mL (1/1000). Immunohistochemistry on Paraffin Sections: 0.5 μg/mL (1/400), Proteinase K pretreatmentfor antigen retrieval is recommended. Positive Control: Human tonsil. Has been discribed to work in FACS with permeabilized cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.5 mL distilled water.

Concentration: 0.2 mg/mL

Buffer: PBS, pH 7.2, containing 5 mg/mL BSA and 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the lyophilised antibody at 2-8 °C and after reconstitution at -20 °C.

Target Details for alpha Defensin 1

Target: alpha Defensin 1 (DEFA1) (Defensin, alpha 1 (DEFA1))

Alternative Name: Defensin alpha 1 (DEFA1 Products)

Synonyms: DEF1 antibody, DEFA2 antibody, HNP-1 antibody, HP-1 antibody, HP1 antibody, MRS antibody, Defcr antibody, Defcr1 antibody, DEFA1 antibody, Defa antibody, Def antibody, GB19392 antibody, defensin alpha 1 antibody, defensin, alpha 1 antibody, defensin alpha 5 antibody, alpha-defensin 1A antibody, defensin 1 antibody, neutrophil defensin 1 antibody, DEFA1 antibody, Defa1 antibody, Defa5 antibody, MNP1A antibody, Def1 antibody, LOC107973256 antibody

Background: Defensins are a family of cyclic peptides containing 29-35 amino acids (MW < 3500) which tend to form aggregates. Four of these peptides are described in humans (HNP-1 to 4), six in rabbits (NP-1 to 5). Synonyms are MCP-1 for NP-1, MCP-2 for NP-2 and corticostatin for NP-3. Biological Functions: Various functions have been described for defensins. They are antibacterial, antifungal, chemotactic for monocytes, inhibitory for ACTH-induced corticosteroid synthesis and cytotoxic for cells. Defensins are inhibited by glucosaminoglycans (self protection for cells) and high concentrations of Ca2+ ions.Synonyms: DEF-1, DEF-3, DEF-A1, DEF-A2, DEF-A3, DEF1, DEF3, DEFA-1, DEFA-2, DEFA-3, DEFA1, DEFA2, DEFA3, Defensin alpha 1, Defensin alpha 3, HNP-1, HNP-3, HNP1, HNP3, HP-1, HP-3, HP1, HP3, MRS, Neutrophil defensin 1, Neutrophil defensin 3

Gene ID: 1667

NCBI Accession: NP_001035965

UniProt: P59665

Pathways: Cellular Response to Molecule of Bacterial Origin