B-Cell CLL/lymphoma 2 (BCL2) antibody

Antigen: B-Cell CLL/lymphoma 2 (BCL2)

Synonyms: Bcl-2, bcl-2, BCL2

Clonality: Monoclonal (100)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN118990

Additional Information

Alternative name: Bcl-2

UniProt: P10415

Immunogen: Synthetic peptide containing aa 41-54 of the bcl-2 protein

Cross-Reactivity: Human

Isotype: IgG1

Clone: 100

Description: Bcl-2 is an integral membrane protein which lies within the cell rather than on the cell surface. The protein is localised in mitochondria and plays a role in the inhibition of apoptosis. Alternate Names: Apoptosis Regulator Bcl-2, BCL2, Bcl-2 family, B-Cell CLL/lymphoma 2, B-Cell Leukemia 2.

Application Details

Application Notes: Immunoprecipitation. Western Blot. Flow Cytometry (1/50-1/100): Membrane permeabilisation is required for this application. Immunohistochemistry on Frozen Sections: (1/20-1/40). Immunohistochemistry on Paraffin Sections: (1/20-1/40): This product requires antigen retrieval using heat treatment prior to staining of formalin fixed material Fixation using BS or Bouins fluid is preferable to formalin fixation. Recommended Positive Control: Human tonsil. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Purification: Purified

Buffer: PBS, pH 7.4 containing 0.09% Sodium Azide as preservative.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Research Area: Cancer, Apoptosis/Necrosis

Restrictions: For Research Use only

Publications

Pezzella, Tse, Cordell et al.: "Expression of the bcl-2 oncogene protein is not specific for the 14;18 chromosomal translocation." in: The American journal of pathology, Vol. 137, Issue 2, pp. 225-32, 1990 (PubMed).

Hockenbery, Nuñez, Milliman et al.: "Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death." in: Nature, Vol. 348, Issue 6299, pp. 334-6, 1991 (PubMed).

Cleary, Smith, Sklar: "Cloning and structural analysis of cDNAs for bcl-2 and a hybrid bcl-2/immunoglobulin transcript resulting from the t(14;18) translocation." in: Cell, Vol. 47, Issue 1, pp. 19-28, 1986 (PubMed).

Tsujimoto, Croce: "Analysis of the structure, transcripts, and protein products of bcl-2, the gene involved in human follicular lymphoma." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, Issue 14, pp. 5214-8, 1986 (PubMed).