BCL2 antibody (B-Cell CLL/lymphoma 2) (AA 41-54)

Details for Product anti-BCL2 Antibody No. ABIN118990, Supplier: Log in to see
Antigen
  • Bcl-2
  • PPP1R50
  • bcl-2
  • AW986256
  • C430015F12Rik
  • D630044D05Rik
  • D830018M01Rik
  • BCL-2
  • Bcl2
  • B-cell CLL/lymphoma 2
  • B cell leukemia/lymphoma 2
  • B-cell leukemia/lymphoma 2
  • B-cell leukemia/lymphoma 2-like
  • B-cell CLL/lymphoma 2 protein
  • BCL2
  • Bcl2
  • LOC100224888
  • bcl2
  • LOC100359045
  • BCL-2
Alternatives
anti-Human BCL2 antibody for Cell Culture
Epitope
AA 41-54
164
73
64
59
41
33
25
25
24
21
18
15
15
15
13
13
13
11
9
8
6
6
5
5
5
5
5
4
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
1001
253
211
54
28
22
18
18
5
4
4
3
2
2
2
2
Host
Mouse
549
517
19
6
2
2
2
Clonality (Clone)
Monoclonal ()
Conjugate
This BCL2 antibody is un-conjugated
49
48
43
37
24
23
22
15
15
13
13
13
13
13
13
13
12
12
12
7
7
7
6
6
6
6
6
6
6
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
790
374
360
309
307
297
227
191
169
81
40
15
14
13
12
11
9
4
3
2
1
1
1
1
1
1
1
Options
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Immunogen Synthetic peptide containing aa 41-54 of the bcl-2 protein.
Clone 100
Isotype IgG1
Specificity This antibody clone 100 reacts specifically with the 25 kDa Bcl-2 oncoprotein. This antibody reacts with small B lymphocytes in the mantle zone and many cells within T cell areas. Very few cells in germinal centres are stained. In the thymus many cells in the medulla are stained but the cortex shows weak or negative staining. In non-haematopoietic tissues few cells are stained. The antibody reacts with neoplastic cells of follicular lymphoma and anaplastic large cell lymphoma.
Purification Affinity chromatography on Protein G
Alternative Name Bcl-2 (BCL2 Antibody Abstract)
Background Human Apoptosis regulator Bcl-2 oncoprotein, a 239 amino acid ~25 kDa integral single pass membrane protein containing 4 BH motifs which lies within the cell rather than on the cell surface. The protein is localised in the outer mitochondrial membrane and plays a role in the inhibition of apoptosis. Bcl-2 is an integral membrane protein which lies within the cell rather than on the cell surface. The protein is localised in mitochondria and plays a role in the inhibition of apoptosis.Synonyms: Apoptosis regulator Bcl-2, B-Cell CLL/lymphoma 2, BCL2, Bcl-2 alpha
Molecular Weight 25-26 kDa
Gene ID 596
NCBI Accession NP_000624
UniProt P10415
Research Area Cancer, Apoptosis/Necrosis
Pathways MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling
Application Notes Immunoprecipitation. Western Blot. Flow Cytometry (1/50-1/100): Membrane permeabilisation is required for this application. Immunohistochemistry on Frozen Sections: (1/20-1/40). Immunohistochemistry on Paraffin Sections: (1/20-1/40): This product requires antigen retrieval using heat treatment prior to staining of formalin fixed material Fixation using BS or Bouins fluid is preferable to formalin fixation. Recommended Positive Control: Human tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4, 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications Hockenbery, Nuñez, Milliman, Schreiber, Korsmeyer: "Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death." in: Nature, Vol. 348, Issue 6299, pp. 334-6, 1991 (PubMed).

Pezzella, Tse, Cordell, Pulford, Gatter, Mason: "Expression of the bcl-2 oncogene protein is not specific for the 14;18 chromosomal translocation." in: The American journal of pathology, Vol. 137, Issue 2, pp. 225-32, 1990 (PubMed).

Tsujimoto, Croce: "Analysis of the structure, transcripts, and protein products of bcl-2, the gene involved in human follicular lymphoma." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, Issue 14, pp. 5214-8, 1986 (PubMed).

Cleary, Smith, Sklar: "Cloning and structural analysis of cDNAs for bcl-2 and a hybrid bcl-2/immunoglobulin transcript resulting from the t(14;18) translocation." in: Cell, Vol. 47, Issue 1, pp. 19-28, 1986 (PubMed).