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Keratin 16 (KRT16) (C-Term) antibody

Details for Product No. ABIN119041
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Antigen
Synonyms KRT17, krt16, CK16, FNEPPK, K16, K1CP, KRT16A, NEPPK, AI324768, Krt1-16, Ka16
Epitope
C-Term
(20), (6), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(97), (45), (43), (14), (14), (1), (1), (1)
Host
Mouse
(73), (23), (3)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(5), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(55), (36), (26), (25), (20), (18), (8), (7), (2), (1)
Pubmed 3 references available
Quantity 0.5 mL
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Catalog No. ABIN119041
253.00 $
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Immunogen Synthetic peptide from the C-terminus of human cytokeratin 16
Clone LL025
Isotype IgG1
Specificity This antibody is specific to a 48 kD protein which is identified as cytokeratin 16. Cytokeratin 16 is expressed in keratinocytes which are undergoing rapid turnover in the suprabasal region. High concordance was found between the carcinomas immunostaining with the basal cell and the hyperproliferation related keratins. Cellular localization: Cytoplasmic
Purification Supernatant
Alternative Name Cytokeratin 16
Background Keratin 16 is expressed in keratinocytes, which are undergoing rapid turnover in the suprabasal region (also known as hyperproliferation-related keratins). Keratin 16 is absent in normal breast tissue and in noninvasive breast carcinomas. Only 10% of the invasive breast carcinomas show diffuse or focal positivity. Reportedly, a relatively high concordance was found between the carcinomas immunostaining with the basal cell and the hyperproliferation-related keratins, but not between these markers and the proliferation marker Ki-67. This supports the conclusion that basal cells in breast cancer may show extensive proliferation, and that absence of Ki-67 staining does not mean that (tumor) cells are not proliferating. Two cytokeratins, 6 and 16, are expressed when keratincytes are undergoing rapid turnover in various pathological states, wound healing, psoriasis and some carcinomas.
Alternate names: CK16, Cytokeratin-16, K16, KRT16, KRT16A, Keratin 16, Keratin type I cytoskeletal 16, Keratin-16
Gene ID 3868
NCBI Accession NP_005548.2
UniProt P08779
Research Area Signaling, Cytoskeleton, Cytokeratins, Extracellular Matrix
Application Notes Immunohistochemistry: This antibody may be diluted to a titer of 1:25-1:75 in an ABC method. We suggest an incubation period of 30 minutes at room temperature. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Recommended positive control: Skin. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Supplier Images
anti-Keratin 16 (KRT16) (C-Term) antibody Formalin fixed paraffin embedded human squamous carcinoma of lung stained with Cytokeratin 16 antibody ABIN119040
Product cited in: Hachiya, Sriwiriyanont, Fujimura et al.: "Mechanistic effects of long-term ultraviolet B irradiation induce epidermal and dermal changes in human skin xenografts." in: The American journal of pathology, Vol. 174, Issue 2, pp. 401-13, 2009 (PubMed).

General Markey, Lane, Macdonald et al.: "Keratin expression in basal cell carcinomas." in: The British journal of dermatology, Vol. 126, Issue 2, pp. 154-60, 1992 (PubMed).

Wetzels, Kuijpers, Lane et al.: "Basal cell-specific and hyperproliferation-related keratins in human breast cancer." in: The American journal of pathology, Vol. 138, Issue 3, pp. 751-63, 1991 (PubMed).

Validation Images
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