Myeloperoxidase antibody (PE)

Catalog No. ABIN119049

Product Details anti-Myeloperoxidase Antibody (PE)

Target: Myeloperoxidase (MPO)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Myeloperoxidase antibody is conjugated to PE

Application: Flow Cytometry (FACS)

No Cross-Reactivity: Rat (Rattus)

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography on Protein G

Immunogen: Human Myeloperoxiase Remarks: Spleen cells from immunised mice were fused with cells of the mouse X63 AG8-653 myeloma cell line.

Clone: 2C7

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: Membrane permeabilisation is required for this application.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1 mL distilled water

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative, 1 % BSA and 5 % sucrose as stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: This product is photosensitive and should be protected from light

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!

References for anti-Myeloperoxidase antibody (PE) (ABIN119049)

Lau, Mollnau, Eiserich, Freeman, Daiber, Gehling, Brümmer, Rudolph, Münzel, Heitzer, Meinertz, Baldus: "Myeloperoxidase mediates neutrophil activation by association with CD11b/CD18 integrins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 2, pp. 431-6, (2005) (PubMed).

Target Details for Myeloperoxidase

Target: Myeloperoxidase (MPO)

Alternative Name: Myeloperoxidase (MPO Products)

Synonyms: mKIAA4033 antibody, MPO antibody, LOC100335032 antibody, POX2' antibody, XPOX2' antibody, mpo antibody, mpo-A antibody, pmr-1 antibody, pmr1 antibody, pox2 antibody, xpox2 antibody, myeloperoxidase antibody, myeloperoxidase L homeolog antibody, MPO antibody, Mpo antibody, LOC100335032 antibody, mpo.L antibody

Background: MPO is an important component of azurophilic granules in neutrophils. Myeloperoxidase is a hemoprotein that is abundantly expressed in neutrophils and secreted during their activation. Native Myeloperoxidase is represented as a covalently bound tetrameric complex of two glycosylated alpha chains (MW 59 - 64 kDa) and two unglycosylated beta chains (MW 14 kDa) with total MW 150 kDa and theoretical pI 9.2. Traditionally Myeloperoxidase was considered as a main target of anti-neutrophil cytoplasm antibodies (ANCA), the serological markers for certain systemic vasculities e.g. periarteriitis nodosa, microscopic polyarteriitis and pulmonary eosinophilic granulomatosis (Churg-Strauss syndrome). Low to moderate anti-Myeloperoxidase autoantibody levels are also reported in rheumatoid arthritis. Recently it was shown that Myeloperoxidase participates in the initiation and progression of cardiovascular disease. It possesses potent proinflammatory properties and may contribute directly to tissue injury. Now Myeloperoxidase is under consideration as one of the most promising cardiac markers.Synonyms: MPO

Gene ID: 9606

UniProt: P05164

Pathways: Chromatin Binding