You are viewing an incomplete version of our website. Please click to reload the website as full version.

RAF1 antibody (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1) (pSer338)

Details for Product anti-RAF1 Antibody No. ABIN119063, Supplier: Log in to see
Antigen
  • c-raf
  • craf
  • raf1
  • RAF1
  • raf
  • CRAF
  • NS5
  • Raf-1
  • c-Raf
  • 6430402F14Rik
  • AA990557
  • BB129353
  • Craf1
  • D830050J10Rik
  • v-Raf
  • AFR1
  • RAF
  • Afr-1
  • Afr1
  • Raf
  • mKIAA0854
  • v-raf-1 murine leukemia viral oncogene homolog 1a
  • v-raf-1 murine leukemia viral oncogene homolog 1
  • raf protein
  • v-raf-leukemia viral oncogene 1
  • zinc fingers and homeoboxes 2
  • raf1a
  • RAF1
  • raf1
  • LOC100341921
  • LOC100347407
  • c-raf
  • Raf1
  • ZHX2
  • Zhx2
Alternatives
anti-Human RAF1 antibody for Enzyme Immunoassay
Epitope
pSer338
71
69
46
37
36
35
28
28
28
27
22
22
21
20
19
18
16
15
15
15
15
15
14
14
10
8
8
7
6
6
6
6
6
4
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
783
403
381
25
23
16
16
13
8
8
8
5
4
2
2
2
2
1
1
1
Host
Rat
750
41
11
6
2
Clonality (Clone)
Monoclonal ()
Conjugate
This RAF1 antibody is un-conjugated
44
44
43
29
29
29
13
13
12
12
12
12
12
12
12
12
12
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
468
343
202
172
156
89
49
32
19
18
17
7
2
2
2
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Immunogen Synthetic peptide GQRDpSSYpYWEIEAS coupled to glutaraldehyde.
Clone P5338 (DPR-2-2A-3A)
Isotype IgG1
Specificity This antibody recognises activated Raf-1, binding to the phosphorylated serine residue at position 338. The antibody binds to Raf-1 that has been activated by Ras or Src, but not to unactivated Raf-1. The antibody does not bind to Raf peptides phosphorylated at other sites, including Y341 and S339.
Purification Affinity chromatography on Protein G
Alternative Name RAF1 (RAF1 Antibody Abstract)
Background Synonyms: C-RAF, RAF proto-oncogene serine/threonine-protein kinase, Raf-1, cRaf
Gene ID 5894
NCBI Accession NP_002871
UniProt P04049
Research Area Signaling, Kinases/Phosphatases
Pathways MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C, Autophagy, Signaling of Hepatocyte Growth Factor Receptor
Application Notes Western Blot: 1/100 - 1/1000, This antibody may be used for Western blotting detection of activated Raf-1 as described by Mason et al. (1999).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-RAF1 antibody (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1) (pSer338) (ABIN119063) Formalin-fixed, paraffin-embedded human tonsil stained with SM1489 (Note nuclear stai...
Background publications Mason, Springer, Cooper, Superti-Furga, Marshall, Marais: "Serine and tyrosine phosphorylations cooperate in Raf-1, but not B-Raf activation." in: The EMBO journal, Vol. 18, Issue 8, pp. 2137-48, 1999 (PubMed).