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V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (pSer338) antibody

Details for Product No. ABIN119063
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Antigen
Synonyms NS5, CRAF, Raf-1, c-Raf, 6430402F14Rik, AA990557, BB129353, Craf1, D830050J10Rik, v-Raf, raf, raf1, c-raf, craf, RAF1
Epitope
pSer338
(63), (46), (31), (30), (25), (24), (24), (24), (24), (22), (19), (17), (17), (17), (16), (16), (15), (12), (12), (12), (8), (8), (7), (7), (6), (6), (6), (6), (6), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(612), (336), (328), (152), (149), (120), (113), (12), (2), (2), (2)
Host
Rat
(571), (33), (14), (8), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(28), (28), (28), (15), (15), (15), (13), (13), (13), (13), (13), (13), (13), (13)
Application
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(388), (305), (141), (130), (101), (96), (27), (16), (11), (11), (2), (2), (1), (1), (1)
Pubmed 1 reference available
Quantity 0.1 mg
Shipping to United States (Change)
Availability Will be delivered in 6 to 8 Business Days
Catalog No. ABIN119063
467.50 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Synthetic peptide GQRDpSSYpYWEIEAS coupled to glutaraldehyde.
Clone P5338 (DPR-2-2A-3A)
Isotype IgG1
Specificity This antibody recognises activated Raf-1, binding to the phosphorylated serine residue at position 338. The antibody binds to Raf-1 that has been activated by Ras or Src, but not to unactivated Raf-1. The antibody does not bind to Raf peptides phosphorylated at other sites, including Y341 and S339.
Purification Affinity chromatography on Protein G
Alternative Name RAF1
Background Alternate names: C-RAF, RAF proto-oncogene serine/threonine-protein kinase, Raf-1, cRaf
Gene ID 5894
NCBI Accession NP_002871.1
UniProt P04049
Research Area Signaling, Kinases/Phosphatases
Application Notes Western Blot: 1/100 - 1/1000, This antibody may be used for Western blotting detection of activated Raf-1 as described by Mason et al. (1999). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS, pH7.4 containing 0.09% Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Supplier Images
anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (pSer338) antibody Formalin-fixed, paraffin-embedded human tonsil stained with SM1489 (Note nuclear staining of mucous epithelium).
Background publications Mason, Springer, Cooper et al.: "Serine and tyrosine phosphorylations cooperate in Raf-1, but not B-Raf activation." in: The EMBO journal, Vol. 18, Issue 8, pp. 2137-48, 1999 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN119063
467.50 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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