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V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) antibody

Antigen

V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1)
Synonyms NS5, CRAF, Raf-1, c-Raf, craf, c-raf, RAF1, MGC165882, raf1, DKFZp469F0314
Clonality Monoclonal (P5338 (DPR-2-2A-3A))
Host
Reactivity
Alternatives

Human
Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
1 reference available
Catalog no. ABIN119063
Quantity 0.1 mg  (1.0 mg/ml)
Price 429.00 $   Plus shipping costs $45.00
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anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) antibody

Additional Information
Application Details Publications Images Alternatives back to top

Alternative name RAF1
UniProt P04049
Immunogen Synthetic peptide GQRDpSSYpYWEIEAS coupled to glutaraldehyde
Cross-Reactivity Human
Isotype IgG1
Clone P5338 (DPR-2-2A-3A)

Application Details
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Application Notes Western Blot: 1/100 - 1/1000, This antibody may be used for Western blotting detection of activated Raf-1 as described by Mason et al. (1999). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Concentration 1.0 mg/ml
Purification Purified
Buffer PBS, pH7.4 containing 0.09% Sodium Azide
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.
Research Area Signaling, Kinases/Phosphatases
Restrictions For Research Use only

Publications
Application Details Images Alternatives back to top

Mason, Springer, Cooper et al.: "Serine and tyrosine phosphorylations cooperate in Raf-1, but not B-Raf activation." in: The EMBO journal, Vol. 18, Issue 8, pp. 2137-48, 1999 (PubMed).

Alternatives
Application Details Publications Images back to top

Alternatives for antigen "V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1)", type "Antibodies"
Hosts Rabbit (268), Mouse (21), Rat (12), Chicken (3), Sheep (1)
Reactivities Human (297), Mouse (Murine) (169), Rat (Rattus) (169), Cow (Bovine) (36), Horse (Equine) (33), Dog (Canine) (31), Chicken (18), Guinea Pig (5), Pig (Porcine) (5), Monkey (1), Xenopus laevis (1)
Applications Western Blotting (WB) (181), ELISA (128), Immunofluorescence (IF) (88), Immunohistochemistry (IHC) (58), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (46), Immunoprecipitation (IP) (12), Dot Blot (Dot) (10), Flow Cytometry (FACS) (3), Immunocytochemistry (ICC) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Dot Blot (DB) (1), Immunoelectron Microscopy (IEM) (1)
Conjugates Alexa Fluor 350 (14), Alexa Fluor 488 (14), Alexa Fluor 555 (14), Alexa Fluor 647 (14), PE,Cy5.5 (14), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1)
Epitopes pSer259 (19), pSer338 (19), Internal Region (14), N-Term (10), pSer43 (9), pSer296 (8), pThr269 (8), pTyr341 (8), pSer471 (7), pSer494 (7), C-Term (6), pSer301 (6), pSer339 (6), pSer642,C-Term (6), Ser338 (5), pSer289 (5), pSer462 (5), Ser259 (4), pTyr340,pSer338 (4), Ser621 (3), pSer338, pTyr341 (3), pSer621 (3), pSer642 (3), Ser233 (2), Ser296 (2), Ser43 (2), Ser471 (2), Thr269 (2), Tyr340 (2), pSer233 (2), pTyr340 (2), pTyr340, pTyr341 (2), Pro302 (1), Ser301 (1), Tyr341 (1), pSer277 (1), pSer299 (1), pSer338, pTyr340 (1), pSer602 (1), pTyr529 (1)