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HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody

Details for Product No. ABIN119227, Supplier: Log in to see
Antigen
  • MHC-G
  • B-F
  • B-F-S04
  • B-F-S05
  • B-F-S06
  • B-F-S07
  • B-FI
  • B-FIV
  • BF2
  • BFa2
  • BFw-03
  • BFw-05
  • BFz-01
Reactivity
Human
337
8
7
3
1
Host
Mouse
207
106
27
6
3
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
40
35
25
21
12
5
3
3
3
3
3
3
3
3
3
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
162
147
138
80
49
37
35
32
31
25
12
7
2
2
1
1
1
1
Supplier
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Immunogen Denatured bacterially expressed recombinant Human HLA G heavy chain
Clone MEM-G-1
Isotype IgG1
Specificity This antibody reacts with denatured soluble and membrane bound HLA G heavy chain.
Cross-Reactivity (Details) Species reactivity (tested):Human.
Purification Affinity Chromatography on Protein A
Alternative Name HLA Class I alpha G / HLA-G (HLAG Antibody Abstract)
Background The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In humans, this complex is referred to as the human leukocyte antigen (HLA) region. HLA G belongs to the HLA class I molecules (HLA Class Ib, nonclassical) and is involved in the presentation of foreign antigens to the immune system. This molecule is expressed on the surface of trophoblast cells and plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T lymphocytes, macrophages and mononuclear cells.Synonyms: HLA class I histocompatibility antigen, HLA-6.0, HLAG, MHC class I antigen G
Molecular Weight 38 kDa.
Gene ID 3135
NCBI Accession NP_002118
UniProt P17693
Pathways
Application Notes Western Blot. Immunohistochemistry on paraffin sections: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections, Sodium citrate buffer pH 6.0 is recommended for this purpose. Recommended Positive Control: Human placenta.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS containing 0.09 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications Menier, Saez, Horejsi et al.: "Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules." in: Human immunology, Vol. 64, Issue 3, pp. 315-26, 2003 (PubMed).

Frumento, Franchello, Palmisano et al.: "Melanomas and melanoma cell lines do not express HLA-G, and the expression cannot be induced by gammaIFN treatment." in: Tissue antigens, Vol. 56, Issue 1, pp. 30-7, 2000 (PubMed).

Fournel, Huc, Aguerre-Girr et al.: "Comparative reactivity of different HLA-G monoclonal antibodies to soluble HLA-G molecules." in: Tissue antigens, Vol. 55, Issue 6, pp. 510-8, 2000 (PubMed).