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HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody

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Antigen
Synonyms MHC-G, B-F, B-F-S04, B-F-S05, B-F-S06, B-F-S07, B-FI, B-FIV, BF2, BFa2, BFw-03, BFw-05, BFz-01
Reactivity
Human
(373), (8), (7), (3), (1)
Host
Mouse
(240), (109), (27), (6), (3), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(46), (41), (30), (22), (13), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(180), (170), (165), (83), (62), (55), (45), (43), (37), (32), (17), (7), (2), (2), (1), (1), (1), (1)
Pubmed 1 reference available
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Quantity 0.1 mg
Shipping to United States ( )
Availability Will be delivered in 6 to 8 Business Days
Immunogen Recombinant human HLA-G denaturated heavy chain
Clone MEM-G-4
Isotype IgG1
Specificity The antibody reacted with denaturated HLA-G heavy chain. The recognized epitope is present only on HLA-G1, -G2 and -G5 Molecules. HLA-G1 and -G2 are membrane bound proteins, HLA-G5 is a soluble protein.
Purification Protein A affinity purified immunoglobulin fraction
Purity > 95 % by SDS-PAGE
Alternative Name HLA Class I alpha G / HLA-G (HLAG Antibody Abstract)
Background HLA G belongs to the HLA class I heavy chain paralogues. It is a heterodimer consisting of a heavy chain (with a1, a2, a3 domains) and a light chain (beta 2 microglobulin). Alternate splicing provides both soluble and membrane bound forms of HLA G, with two soluble isoforms and four membrane bound isoforms. HLA G may be part of the fetal protection system and is expressed on fetal derived placental cells.Synonyms: HLA class I histocompatibility antigen, HLA-6.0, HLAG, MHC class I antigen G
Gene ID 3135
NCBI Accession NP_002118
UniProt P17693
Application Notes Western Blotting (MWs: HLA-G1 approx. 45 kDa, HLA-G2 approx. 45 kDa, HLA-G5 approx. 28 kDa).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline pH 7.4 with 15 mM sodium azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Prior to reconstitution store at -70 °C. Following reconstitution store the antibody (in aliquots) at -20 °C for 6 month.
Expiry Date 6 months
Background publications Menier, Saez, Horejsi et al.: "Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules." in: Human immunology, Vol. 64, Issue 3, pp. 315-26, 2003 (PubMed).

Catalog No. ABIN119235
$324.50
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