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HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody

Details for Product No. ABIN119235, Supplier: Log in to see
Antigen
  • MHC-G
  • B-F
  • B-F-S04
  • B-F-S05
  • B-F-S06
  • B-F-S07
  • B-FI
  • B-FIV
  • BF2
  • BFa2
  • BFw-03
  • BFw-05
  • BFz-01
Reactivity
Human
337
8
7
3
1
Host
Mouse
207
106
27
6
3
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
40
35
25
21
12
5
3
3
3
3
3
3
3
3
3
1
1
1
1
1
1
Application
Western Blotting (WB)
162
147
138
80
49
37
35
32
32
25
12
7
2
2
1
1
1
1
Supplier
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Immunogen Recombinant human HLA-G denaturated heavy chain
Clone MEM-G-4
Isotype IgG1
Specificity The antibody reacted with denaturated HLA-G heavy chain. The recognized epitope is present only on HLA-G1, -G2 and -G5 Molecules. HLA-G1 and -G2 are membrane bound proteins, HLA-G5 is a soluble protein.
Purification Protein A affinity purified immunoglobulin fraction
Purity > 95 % by SDS-PAGE
Alternative Name HLA Class I alpha G / HLA-G (HLAG Antibody Abstract)
Background HLA G belongs to the HLA class I heavy chain paralogues. It is a heterodimer consisting of a heavy chain (with a1, a2, a3 domains) and a light chain (beta 2 microglobulin). Alternate splicing provides both soluble and membrane bound forms of HLA G, with two soluble isoforms and four membrane bound isoforms. HLA G may be part of the fetal protection system and is expressed on fetal derived placental cells.Synonyms: HLA class I histocompatibility antigen, HLA-6.0, HLAG, MHC class I antigen G
Gene ID 3135
NCBI Accession NP_002118
UniProt P17693
Pathways
Application Notes Western Blotting (MWs: HLA-G1 approx. 45 kDa, HLA-G2 approx. 45 kDa, HLA-G5 approx. 28 kDa).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline pH 7.4 with 15 mM sodium azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Prior to reconstitution store at -70 °C. Following reconstitution store the antibody (in aliquots) at -20 °C for 6 month.
Expiry Date 6 months
Background publications Menier, Saez, Horejsi et al.: "Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules." in: Human immunology, Vol. 64, Issue 3, pp. 315-26, 2003 (PubMed).