CD147 antibody

Catalog No. ABIN119254

Product Details anti-CD147 Antibody

Target: CD147 (BSG) (Basigin (Ok Blood Group) (BSG))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD147 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Specificity: This antibody recognises the CD147 cell surface antigen.

Purification: Affinity Chromatography on Protein G

Immunogen: CD147Rg, consisting of the cDNA coding for the hinge region, CH2 and CH3 domains of Human IgG1.

Clone: MEM-M6-1

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: 1/50 - 1/100, use 10 μL of the suggested working dilution to label 10^6 cells. Immunoprecipitation. Western Blot: under non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-CD147 antibody (ABIN119254)

Schneiderhan, Scheler, Holzmann, Marx, Gschwend, Bucholz, Gress, Seufferlein, Adler, Oswald: "CD147 silencing inhibits lactate transport and reduces malignant potential of pancreatic cancer cells in in vivo and in vitro models." in: Gut, Vol. 58, Issue 10, pp. 1391-8, (2009) (PubMed).

Target Details for CD147

Target: CD147 (BSG) (Basigin (Ok Blood Group) (BSG))

Alternative Name: CD147 (BSG Products)

Synonyms: EMMPRIN antibody, RPE7 antibody, 5F7 antibody, CD147 antibody, M6 antibody, OK antibody, TCSF antibody, AI115436 antibody, AI325119 antibody, HT-7 antibody, 5A11 antibody, CE9 antibody, HT7 antibody, Ox47R antibody, basignin antibody, wu:fb26a05 antibody, wu:fo98e12 antibody, bsg antibody, prso03 antibody, basigin (Ok blood group) antibody, basigin antibody, basigin (Ok blood group) S homeolog antibody, BSG antibody, Bsg antibody, bsg antibody, bsg.S antibody

Background: CD147 is a 50-60kD glycoprotein also known as neurothelin and basigin. CD147 is broadly expressed on peripheral blood cells and by endothelial cells. Expression upon T cells is increased following activation.Synonyms: 5F7, BSG, Basigin, Collagenase stimulatory factor, EMMPRIN, Extracellular matrix metalloproteinase inducer, Leukocyte activation antigen M6, OK blood group antigen, TCSF, Tumor cell-derived collagenase stimulatory factor

Gene ID: 682

NCBI Accession: NP_001719

UniProt: P35613

Pathways: S100 Proteins