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Huntingtin (HTT) (AA 997-1276) antibody

Details for Product No. ABIN119305
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Antigen
Synonyms HD, IT15, Hd, Hdh, AI256365, C430023I11Rik, SLC6A4, HTT, hd, ZHD, CG9995, Dmel\\CG9995, Hsap\\HD, Htt, dHtt, dhtt, huntington, it15, htt
Epitope
AA 997-1276
(17), (12), (7), (5), (5), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rabbit
(96), (41), (38), (13), (13), (12), (12), (3), (3)
Host
Mouse
(53), (46), (2), (1), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
(78), (60), (31), (25), (22), (16), (15), (10), (7), (3), (2), (1), (1)
Pubmed 2 references available
Quantity 0.1 mg
Shipping to United States (Change)
Availability Will be delivered in 6 to 8 Business Days
Catalog No. ABIN119305
533.50 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Recombinant protein corresponding to amino acids 997 -1276 of huntingtin
Clone HDA3E10
Isotype IgG2a
Specificity This antibody reacts with an epitope corresponding to the HDA region (amino acids 1171 - 1177) of the huntingtin protein. Clone HDA3E10 detects a 350KD band on western blots but also detects smaller degradation products of huntingtin. The combined use of clone HDA3E10 (SM1660) HDC8A4 (SM1662) and HDB4E10 (SM1661) demonstrate that huntingtin is enriched in neuronal cells in the brain.
Purification Purified
Alternative Name Huntingtin
Background Huntingtin is a 350kDa protein of 3144 amino acids that is altered in Huntington's disease (HD). The expanded trinucleotide CAG repeat of the HD gene encodes an abnormally expanded polyglutamine stretch in the N-terminus of the Huntingtin protein. The normal function of Huntingtin still remains uncertain but it has been suggested that it performs a necessary housekeeping function. The abnormal form of Huntingtin aggregates in vitro and forms neuronal intranuclear and cytoplasmic inclusions in HD patients. The expanded polyglutamine repeats formed by this mutation of the Huntingtin gene have been proposed to cause neuronal degeneration in HD through abnormal interactions with other proteins containing short polyglutamine tracts such as the CREB binding protein (CBP), a protein that promotes cell survival and is a major mediator of survival signals in mature neurons.
Alternate names: HD, HTT, Huntington Disease Protein, IT15
Gene ID 3064
NCBI Accession NP_002102.4
UniProt P42858
Research Area Neurology, Virology
Application Notes Western blotting. Immunoprecipitation. Immunohistochemistry on frozen sections, recommended positive control tissue: Brain (Increased cytoplasmic staining, relative to nuclear, has been reported using formaldehyde as a fixative compared with acetone/methanol(1). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS buffer with 0.09% sodium azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Supplier Images
anti-Huntingtin (HTT) (AA 997-1276) antibody Total protein extract of normal human cerebral cortex separated as a strip on a 3-12.5% gradient SDS-PAGE gel and Western blotted. The blot was probed with ABIN119305
Background publications Wilkinson, Nguyen, Manilal et al.: "Localization of rabbit huntingtin using a new panel of monoclonal antibodies." in: Brain research. Molecular brain research, Vol. 69, Issue 1, pp. 10-20, 1999 (PubMed).

Nucifora, Sasaki, Peters et al.: "Interference by huntingtin and atrophin-1 with cbp-mediated transcription leading to cellular toxicity." in: Science (New York, N.Y.), Vol. 291, Issue 5512, pp. 2423-8, 2001 (PubMed).

Hosts (53), (46), (2), (1), (1)
Reactivities (96), (41), (38), (13), (13), (12), (12), (3), (3)
Applications (78), (60), (31), (25), (22), (16), (15), (10), (7), (3), (2), (1), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (17), (12), (7), (5), (5), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN119305
533.50 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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