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Huntingtin antibody (HTT) (AA 997-1276)

Details for Product anti-HTT Antibody No. ABIN119305, Supplier: Login to see
Antigen
  • HTT
  • hd
  • ZHD
  • CG9995
  • Dmel\\CG9995
  • HD
  • Hsap\\HD
  • Htt
  • dHtt
  • dhtt
  • SLC6A4
  • huntington
  • it15
  • htt
  • IT15
  • AI256365
  • C430023I11Rik
  • Hd
  • Hdh
Alternatives
anti-Human Huntingtin antibody for Western Blotting
Epitope
AA 997-1276
13
13
12
7
6
6
6
5
5
5
4
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rabbit
128
46
35
6
1
Host
Mouse
68
64
2
1
Clonality (Clone)
Monoclonal ()
Conjugate
This Huntingtin antibody is un-conjugated
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
101
66
42
23
20
20
12
12
11
8
3
3
2
Supplier
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Immunogen Recombinant protein corresponding to amino acids 997 -1276 of huntingtin
Clone HDA3E10
Isotype IgG2a
Specificity This antibody reacts with an epitope corresponding to the HDA region (amino acids 1171 - 1177) of the huntingtin protein. Clone HDA3E10 detects a 350KD band on western blots but also detects smaller degradation products of huntingtin. The combined use of clone HDA3E10 (SM1660) HDC8A4 (SM1662) and HDB4E10 (SM1661) demonstrate that huntingtin is enriched in neuronal cells in the brain.
Purification Purified
Alternative Name Huntingtin (HTT Antibody Abstract)
Background Huntingtin is a 350 kDa protein of 3144 amino acids that is altered in Huntington's disease (HD). The expanded trinucleotide CAG repeat of the HD gene encodes an abnormally expanded polyglutamine stretch in the N-terminus of the Huntingtin protein. The normal function of Huntingtin still remains uncertain but it has been suggested that it performs a necessary housekeeping function. The abnormal form of Huntingtin aggregates in vitro and forms neuronal intranuclear and cytoplasmic inclusions in HD patients. The expanded polyglutamine repeats formed by this mutation of the Huntingtin gene have been proposed to cause neuronal degeneration in HD through abnormal interactions with other proteins containing short polyglutamine tracts such as the CREB binding protein (CBP), a protein that promotes cell survival and is a major mediator of survival signals in mature neurons.Synonyms: HD, HTT, Huntington Disease Protein, IT15
Gene ID 3064
NCBI Accession NP_002102
UniProt P42858
Research Area Neurology, Virology
Pathways PI3K-Akt Signaling
Application Notes Western blotting. Immunoprecipitation. Immunohistochemistry on frozen sections, recommended positive control tissue: Brain (Increased cytoplasmic staining, relative to nuclear, has been reported using formaldehyde as a fixative compared with acetone/methanol(1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS buffer with 0.09 % sodium azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Western Blotting (WB) image for anti-Huntingtin antibody (HTT) (AA 997-1276) (ABIN119305) Total protein extract of normal human cerebral cortex separated as a strip on a 3-12....
Background publications Nucifora, Sasaki, Peters et al.: "Interference by huntingtin and atrophin-1 with cbp-mediated transcription leading to cellular toxicity." in: Science (New York, N.Y.), Vol. 291, Issue 5512, pp. 2423-8, 2001 (PubMed).

Wilkinson, Nguyen, Manilal et al.: "Localization of rabbit huntingtin using a new panel of monoclonal antibodies." in: Brain research. Molecular brain research, Vol. 69, Issue 1, pp. 10-20, 1999 (PubMed).