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Toll-Like Receptor 4 (TLR4) antibody (Biotin)

Antigen

Toll-Like Receptor 4 (TLR4)
Synonyms TOLL, CD284, hToll, ARMD10, Lps, Ly87, Ran/M1, Rasl2-8
Clonality Monoclonal (HTA125)
Host
Alternatives

Mouse
Reactivity
Alternatives

Dog (Canine), Guinea Pig, Human, Monkey, Pig (Porcine)
Conjugate
Alternatives Biotin
Application
Alternatives Flow Cytometry (FACS)
2 references available
Catalog no. ABIN119328
Quantity 0.1 mg  (0.1 mg/ml)
Price 429.00 $   Plus shipping costs $45.00
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Availability Will be delivered in 6 to 8 Business Days

Additional Information
Application Details Publications Alternatives back to top

Alternative name CD284 / TLR4
UniProt O00206
Immunogen Ba/F3 cell line expressing TLR4 (CD284). Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Cross-Reactivity Dog (Canine), Guinea Pig, Human, Monkey, Pig (Porcine)
Isotype IgG2a
Clone HTA125
Description TLR4, also known as CD284, has been demonstrated to act as a receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 molecule. TLR4 is weakly expressed by resting cells, but is upregulated following stimulation with LPS. Alternate Names: TLR-4, hToll, Toll-like Receptor 4.

Application Details
Publications Alternatives back to top

Application Notes Flow cytometry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Concentration 0.1 mg/ml
Buffer PBS containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.
Research Area Signaling, Immunology, Inflammation
Restrictions For Research Use only

Publications
Application Details Alternatives back to top

Shimazu, Akashi, Ogata et al.: "MD-2, a molecule that confers lipopolysaccharide responsiveness on Toll-like receptor 4." in: The Journal of experimental medicine, Vol. 189, Issue 11, pp. 1777-82, 1999 (PubMed).

Jiang, Akashi, Miyake et al.: "Lipopolysaccharide induces physical proximity between CD14 and toll-like receptor 4 (TLR4) prior to nuclear translocation of NF-kappa B." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 7, pp. 3541-4, 2000 (PubMed).

Alternatives
Application Details Publications back to top

Alternatives for antigen "Toll-Like Receptor 4 (TLR4)", type "Antibodies"
Hosts Rabbit (97), Mouse (91), Rat (10), Goat (4)
Reactivities Human (170), Mouse (Murine) (55), Rat (Rattus) (32), Pig (Porcine) (29), Dog (Canine) (26), Cow (Bovine) (24), Sheep (Ovine) (19), Monkey (12), Guinea Pig (8), Chimpanzee (3), Horse (Equine) (3), Macaque (2), Bison (Bison) (1)
Applications Flow Cytometry (FACS) (102), Western Blotting (WB) (95), ELISA (44), Immunoprecipitation (IP) (35), Immunohistochemistry (IHC) (25), Immunofluorescence (IF) (24), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (22), Immunocytochemistry (ICC) (19), Functional Studies (Func) (13), Neutralization (Neut) (7), Immunohistochemistry (Fixed) (IHC (fx)) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Blocking Antibody (Inhibition) (1), Dot Blot (Dot) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
Conjugates FITC (20), Biotin (16), PE (13), Alexa Fluor (4), Alexa Fluor 647 (4), APC (2), Alexa Fluor 488 (2), RPE (2), Alexa Fluor 350 (1), Alexa Fluor 555 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Cy7,PE (1), Gold (1), HRP (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)
Epitopes N-Term (16), AA 100-200 (11), C-Term (8), Internal Region (6), AA 420-435 (5), AA 650-700 (5), AA 39-56 (2), Center (2), AA 100-150 (1), AA 161-192 (1), AA 6-169 (1), AA 976-998 (1), Extracellular Domain (1), Internal Region,AA 676-706 (1), pTyr674 (1)