TLR4 antibody (Toll-Like Receptor 4)

Details for Product anti-TLR4 Antibody No. ABIN119330, Supplier: Log in to see
Antigen
  • ARMD10
  • CD284
  • TLR-4
  • TOLL
  • Lps
  • Ly87
  • Ran/M1
  • Rasl2-8
  • toll-like receptor 4
  • TLR4
  • Tlr4
Alternatives
anti-Human TLR4 antibody for Immunofluorescence (fixed cells)
Reactivity
Dog (Canine), Guinea Pig, Human, Monkey, Pig (Porcine)
500
258
142
74
50
50
42
26
12
10
4
2
2
1
1
Host
Mouse
292
251
60
9
Clonality (Clone)
Monoclonal ()
Conjugate
This TLR4 antibody is un-conjugated
47
42
40
19
15
13
12
11
10
8
8
8
8
7
7
6
6
6
6
4
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Functional Studies (Func), Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
365
292
136
134
123
107
98
86
34
27
26
23
21
16
12
8
8
4
4
3
3
2
2
1
1
1
Options
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Available images

Immunogen Ba/F3 cell line expressing TLR4 (CD284). Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Clone HTA125
Isotype IgG2a
Purification Affinity chromatography on Protein G
Endotoxin Level < 0.01 EU/ug
Alternative Name CD284 / TLR4 (TLR4 Antibody Abstract)
Background TLR4, also known as CD284, has been demonstrated to act as a receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 Molecule. TLR4 is weakly expressed by resting cells, but is upregulated following stimulation with LPS.Synonyms: Toll-like receptor 4
NCBI Accession NM_138554
UniProt O00206
Research Area Signaling, Immunology, Inflammation
Pathways TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades
Application Notes Flow Cytometry: 1/10 - 1/50, Use 10 μL of the suggested working dilution to label 10^6 cells or 100 μL whole blood. Immunoprecipitation. Functional Assays.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: Endotoxin Level: less than 0.01 EU/ug
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS, None, None
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Supplier Images
Flow Cytometry (FACS) image for anti-TLR4 antibody (Toll-Like Receptor 4) (ABIN119330) Staining of human peripheral blood monocytes with MOUSE ANTI HUMAN CD284: LOW ENDOTOX...
Background publications Jiang, Akashi, Miyake, Petty: "Lipopolysaccharide induces physical proximity between CD14 and toll-like receptor 4 (TLR4) prior to nuclear translocation of NF-kappa B." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 7, pp. 3541-4, 2000 (PubMed).

Shimazu, Akashi, Ogata, Nagai, Fukudome, Miyake, Kimoto: "MD-2, a molecule that confers lipopolysaccharide responsiveness on Toll-like receptor 4." in: The Journal of experimental medicine, Vol. 189, Issue 11, pp. 1777-82, 1999 (PubMed).