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TLR4 antibody (Toll-Like Receptor 4)

Details for Product anti-TLR4 Antibody No. ABIN119332, Supplier: Log in to see
Antigen
  • ARMD10
  • CD284
  • Lps
  • Ly87
  • Ran/M1
  • Rasl2-8
  • TLR-4
  • TOLL
Alternatives
anti-Dog (Canine) TLR4 antibody for Flow Cytometry
Reactivity
Dog (Canine), Guinea Pig, Human, Monkey, Pig (Porcine)
475
201
119
63
43
42
34
24
9
7
3
1
Host
Mouse
263
247
55
10
Clonality (Clone)
Monoclonal ()
Conjugate
This TLR4 antibody is un-conjugated
47
43
40
15
12
11
11
8
8
8
7
7
6
6
6
6
5
4
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
364
277
125
123
120
104
95
89
45
32
24
24
16
12
11
8
4
4
3
2
1
1
1
1
1
Supplier
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Immunogen Ba/F3 cell line expressing TLR4. Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Clone HTA125
Isotype IgG2a
Purification Affinity Chromatography on Protein G
Alternative Name CD284 / TLR4 (TLR4 Antibody Abstract)
Background TLR4, also known as CD284, has been demonstrated to act as a receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 Molecule. TLR4 is weakly expressed by resting cells, but is upregulated following stimulation with LPS.Synonyms: Toll-like receptor 4
NCBI Accession NM_138554
UniProt O00206
Research Area Signaling, Immunology, Inflammation
Pathways TLR Signaling
Application Notes Flow cytometry: 1/10-1/25, use 10 μL of the suggested working solution to label 10^6 cells or 100 μL whole blood. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS containing 0.09 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications de Kleer, Vercoulen, Klein, Meerding, Albani, van der Zee, Sawitzki, Hamann, Kuis, Prakken: "CD30 discriminates heat shock protein 60-induced FOXP3+ CD4+ T cells with a regulatory phenotype." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 185, Issue 4, pp. 2071-9, 2010 (PubMed).

Brännström, Sellin, Holmfeldt, Brattsand, Gullberg: "The Schistosoma mansoni protein Sm16/SmSLP/SmSPO-1 assembles into a nine-subunit oligomer with potential To inhibit Toll-like receptor signaling." in: Infection and immunity, Vol. 77, Issue 3, pp. 1144-54, 2009 (PubMed).

Devaney, Greene, Taggart, Carroll, ONeill, McElvaney: "Neutrophil elastase up-regulates interleukin-8 via toll-like receptor 4." in: FEBS letters, Vol. 544, Issue 1-3, pp. 129-32, 2003 (PubMed).

Bieback, Lien, Klagge, Avota, Schneider-Schaulies, Duprex, Wagner, Kirschning, Ter Meulen, Schneider-Schaulies: "Hemagglutinin protein of wild-type measles virus activates toll-like receptor 2 signaling." in: Journal of virology, Vol. 76, Issue 17, pp. 8729-36, 2002 (PubMed).

Triantafilou, Miyake, Golenbock, Triantafilou: "Mediators of innate immune recognition of bacteria concentrate in lipid rafts and facilitate lipopolysaccharide-induced cell activation." in: Journal of cell science, Vol. 115, Issue Pt 12, pp. 2603-11, 2002 (PubMed).

Yang, Tamai, Akashi, Takeuchi, Akira, Sugawara, Takada: "Synergistic effect of muramyldipeptide with lipopolysaccharide or lipoteichoic acid to induce inflammatory cytokines in human monocytic cells in culture." in: Infection and immunity, Vol. 69, Issue 4, pp. 2045-53, 2001 (PubMed).

Kawahara, Teshima, Oka, Sugiyama, Kishi, Rokutan: "Type I Helicobacter pylori lipopolysaccharide stimulates toll-like receptor 4 and activates mitogen oxidase 1 in gastric pit cells." in: Infection and immunity, Vol. 69, Issue 7, pp. 4382-9, 2001 (PubMed).

Jiang, Akashi, Miyake, Petty: "Lipopolysaccharide induces physical proximity between CD14 and toll-like receptor 4 (TLR4) prior to nuclear translocation of NF-kappa B." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 7, pp. 3541-4, 2000 (PubMed).

Shimazu, Akashi, Ogata, Nagai, Fukudome, Miyake, Kimoto: "MD-2, a molecule that confers lipopolysaccharide responsiveness on Toll-like receptor 4." in: The Journal of experimental medicine, Vol. 189, Issue 11, pp. 1777-82, 1999 (PubMed).