His Tag antibody

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Antigen
  • AKR1B11
  • AKR1B12
  • ALDRLn
  • ARL-1
  • ARL1
  • HIS
  • HSI
  • aldo-keto reductase family 1, member B10 (aldose reductase)
  • AKR1B10
Reactivity
Tag
620
18
14
3
1
Host
Mouse
412
181
27
23
4
4
3
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
59
45
36
15
13
11
10
9
9
8
8
8
8
8
8
7
7
7
6
5
4
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
545
386
163
106
77
76
68
25
12
10
9
7
7
6
6
5
4
4
4
3
2
2
1
1
1
1
1
1
1
1
1
1
Options
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Immunogen PAX6 transcription factor linked to Histidine tag. Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS1 myeloma cell line.
Sequence HHHHHH
Clone AD1-1-10
Isotype IgG1
Purification Affinity Chromatography on Protein G
Alternative Name 6xHistidine Epitope Tag
Target Type Tag
Background The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Application Notes Immunhistology on Frozen Sections. ELISA. Western Blot. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4, containing 0.09 % Sodium Azide as a preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications Bahi, Boyer, Kafri, Dreyer: "CD81-induced behavioural changes during chronic cocaine administration: in vivo gene delivery with regulatable lentivirus." in: The European journal of neuroscience, Vol. 19, Issue 6, pp. 1621-33, 2004 (PubMed).

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