Interleukin 2 (IL2) antibody
| Antigen |
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| Synonyms |
IL-2, TCGF, lymphokine, Il-2, IL2, POIL2, il2 |
| Clonality |
Monoclonal (JES6-1A12) |
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Host
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Reactivity
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Human (57), Mouse (Murine) (35), Rat (Rattus) (8), Pig (Porcine) (5), Cow (Bovine) (4), Monkey (4), Sheep (Ovine) (4), Chicken (2), Bird (Avian) (1), Cat (Feline) (1), Mammalian (1)
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Application
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 Alternatives Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
Enzyme Immunoassay (EIA) (51), Western Blotting (WB) (45), ELISA (35), Functional Studies (Func) (20), Flow Cytometry (FACS) (19), Blocking Antibody (Inhibition) (5), Dot Blot (Dot) (5), Immunohistochemistry (Frozen Sections) (IHC (fro)) (5), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (5), Immunoprecipitation (IP) (5), Immunofluorescence (IF) (4), Radioimmunoassay (RIA) (4), Immunohistochemistry (IHC) (3), Neutralization (Neut) (3), ELISA (Capture) (2), ELISA (Detection) (1)
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| Catalog no. |
ABIN119368 |
| Quantity |
0.1 mg (1.0 mg/ml) (Variants) |
| Price |
215.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Alternative name
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Interleukin-2 / IL2
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Swiss-Prot
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P04351
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Immunogen
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E. coli expressed recombinant mouse IL-2
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Isotype
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IgG2a (Matching secondary antibodies)
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Clone
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JES6-1A12
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Specificity
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This antibody recognises natural and recombinant IL-2.
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Application Notes
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ELISA (as capture antibody, see 1,2): 1-4 ug/ml, apropriate range of IL-2 concentrations for linear standard curve is 2 ng/ml. Immunoprecipitation. Western blotting (1), for the latter, 1-5 ug/ml of this clone were found to detect less than 100 ng per lane of recombinant mouse IL-2 under reducing conditions. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Concentration
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1.0 mg/ml
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Buffer
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PBS containing 0.02 % sodium azide
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Storage
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Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.
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Restrictions
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For Research Use only
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Publications
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Abrams, Roncarolo, Yssel et al.: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, pp. Mai 24, 1992 (PubMed).
Sander, Hoeiduen, Andersson et al.: "Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining." in: Journal of immunological methods, Vol. 166, Issue 2, pp. 201-14, 1994 (PubMed).
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Alternatives for antigen "Interleukin 2 (IL2)", type "Antibodies"
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Hosts
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Mouse (49), Rabbit (33), Rat (33), Goat (5), Chicken (1)
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Reactivities
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Human (57), Mouse (Murine) (35), Rat (Rattus) (8), Pig (Porcine) (5), Cow (Bovine) (4), Monkey (4), Sheep (Ovine) (4), Chicken (2), Bird (Avian) (1), Cat (Feline) (1), Mammalian (1)
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Applications
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Enzyme Immunoassay (EIA) (51), Western Blotting (WB) (45), ELISA (35), Functional Studies (Func) (20), Flow Cytometry (FACS) (19), Blocking Antibody (Inhibition) (5), Dot Blot (Dot) (5), Immunohistochemistry (Frozen Sections) (IHC (fro)) (5), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (5), Immunoprecipitation (IP) (5), Immunofluorescence (IF) (4), Radioimmunoassay (RIA) (4), Immunohistochemistry (IHC) (3), Neutralization (Neut) (3), ELISA (Capture) (2), ELISA (Detection) (1)
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Conjugates
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Biotin (14), FITC (5), PE (2), RPE (2), HRP (1)
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Epitopes
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Biotinylated (3), Center (1)
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