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Estrogen Receptor alpha antibody (Estrogen Receptor 1)

Details for Product anti-ESR1 Antibody No. ABIN119395, Supplier: Log in to see
Antigen
  • ACTGP1
  • ACT1GP1
  • HY-psi-gamma-AC6
  • ER[b]2
  • ER[b]a
  • zfER[b]2
  • zfER-beta2
  • XER
  • era
  • esr
  • esra
  • nr3a1
  • ERbeta
  • xesr-1
  • ERalpha
  • xlERalpha1
  • xlERalpha2
  • LOC398733
  • LOC398734
  • 2610524P08Rik
  • 9130407C09Rik
  • AU019798
  • Era
  • M-ERA
  • MERA-S
  • MERA-W
  • ERA
  • ERAL1A
  • H-ERA
  • HERA-A
  • HERA-B
  • er
  • esr1
  • ER
  • ESR
  • ESRA
  • ESTRR
  • NR3A1
  • ER-alpha
  • Esr
  • RNESTROR
  • ERALPHA
  • ER[a]
  • eralpha
  • zfER[a]
  • ESR1
  • AA420328
  • AU041214
  • ERa
  • Estr
  • Estra
  • Nr3a1
  • actin, gamma 1 pseudogene 1
  • estrogen receptor 2a
  • estrogen receptor 1
  • estrogen receptor alpha 2
  • estrogen receptor alpha 1
  • Era (G-protein)-like 1 (E. coli)
  • Era-like 12S mitochondrial rRNA chaperone 1
  • estrogen receptor
  • estrogen receptor 1 (alpha)
  • estrogen receptor alpha-like
  • ACTG1P1
  • esr2a
  • esr1
  • LOC398733
  • LOC398734
  • Eral1
  • ERAL1
  • er
  • ESR1
  • Esr1
  • LOC100351261
Alternatives
anti-Human Estrogen Receptor alpha antibody for Immunohistochemistry (Paraffin-embedded Sections)
Reactivity
Human
1112
380
270
58
51
26
19
14
12
11
9
3
2
2
1
1
1
Host
Mouse
681
467
8
3
2
Clonality (Clone)
Monoclonal ()
Conjugate
This Estrogen Receptor alpha antibody is un-conjugated
42
40
39
27
19
19
15
15
12
11
11
11
11
11
11
11
11
10
10
8
8
8
8
8
8
8
8
8
6
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
884
408
362
294
168
106
106
92
84
64
15
12
9
9
7
7
6
4
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Options
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Immunogen Prokaryotic recombinant protein corresponding to the full-length estrogen receptor molecule.
Clone 6F11
Isotype IgG1
Specificity This antibody is specific to estrogen receptor, which is associated with superior prognosis and a better response to endocrine therapy.
Cellular Localization: Nuclear
Purification Supernatant
Alternative Name Estrogen Receptor alpha (ESR1 Antibody Abstract)
Background The nuclei of the estrogen receptor positive cells stain very strongly with this antibody, without any staining in the cytoplasm.Synonyms: ER alpha, ESR, ESR1, Estradiol receptor, NR3A1, Nuclear receptor subfamily 3 group A member 1
Gene ID 2099
NCBI Accession NP_000116
UniProt P03372
Research Area Cancer, Endocrine system, Transcription Factors, Hormones, Receptors
Pathways Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway
Application Notes Immunoprecipitation: 1:50. Western Blot: 1:50 to 1:100. Immunohistochemistry on formalin-fixed paraffin embedded sections: diluted of 1:20 to 1:80 in an ABC method (30 minutes at room temperature) Recommended Positive Control: Breast carcinoma
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer 10 mM citrate buffer, pH 6.0 - containing sodium azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Product cited in: Konwisorz, Springwald, Haselberger, Goerse, Ortmann, Treeck: "Knockdown of ICB-1 gene enhanced estrogen responsiveness of ovarian and breast cancer cells." in: Endocrine-related cancer, Vol. 17, Issue 1, pp. 147-57, 2010 (PubMed).

Background publications Bur, Zimarowski, Schnitt, Baker, Lew: "Estrogen receptor immunohistochemistry in carcinoma in situ of the breast." in: Cancer, Vol. 69, Issue 5, pp. 1174-81, 1992 (PubMed).

Graham, Jin, Lloyd: "Detection of estrogen receptor in paraffin-embedded sections of breast carcinoma by immunohistochemistry and in situ hybridization." in: The American journal of surgical pathology, Vol. 15, Issue 5, pp. 475-85, 1991 (PubMed).

Pertschuk, Kim, Nayer, Feldman, Eisenberg, Carter, Rong, Thelmo, Fleisher, Greene: "Immunocytochemical estrogen and progestin receptor assays in breast cancer with monoclonal antibodies. Histopathologic, demographic, and biochemical correlations and relationship to endocrine response and survival." in: Cancer, Vol. 66, Issue 8, pp. 1663-70, 1990 (PubMed).

Kumar, Green, Stack, Berry, Jin, Chambon: "Functional domains of the human estrogen receptor." in: Cell, Vol. 51, Issue 6, pp. 941-51, 1988 (PubMed).