Estrogen Receptor 1 (ESR1) antibody

Antigen: Estrogen Receptor 1 (ESR1)

Synonyms: AAC2, PNAT, ACTGP1, ACT1GP1, HY-psi-gamma-AC6, 7B4, CD144, FLJ17376, ER, ESR, Era, ESRA, NR3A1, DKFZp686N23123, ERa, Estr, ER[a], Estra, Nr3a1, ERalpha, AA420328, AU041214, ER-alpha, ER[b], Estrb, ERbeta, Esr, RNESTROR, eralpha, zfER[a], ESR2, ERB, Errra, ERRalpha, ER[b]1, zfER[b]1, MGC103724, zfER-beta1, ER[b]2, ER[b]a, zfER[b]2, zfER-beta2, erb, esrb, estrb, nr3a2, er-beta, esr-beta, XER, era, esr, esra, nr3a1, xesr-1, xlERalpha1, xlERalpha2, CERB, ER-beta, ESR1, LOC398733, LOC398734, ESRRA, ESTRRA, ERBETA, ERALPHA, err1, erra, esrral, erralpha, esrl1, nr3b1, MGC147343, er, LOC100125507

Clonality: Monoclonal (6F11)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN119395

Additional Information

Alternative name: Estrogen receptor alpha

UniProt: P03372

Immunogen: Prokaryotic recombinant protein corresponding to the full-length estrogen receptor molecule

Cross-Reactivity: Human

Isotype: IgG1

Clone: 6F11

Description: The nuclei of the estrogen receptor positive cells stain very strongly with this antibody, without any staining in the cytoplasm.

Synonyms: AAC2, PNAT, ACTGP1, ACT1GP1, HY-psi-gamma-AC6, 7B4, CD144, FLJ17376, ER, ESR, Era, ESRA, NR3A1, DKFZp686N23123, ERa, Estr, ER[a], Estra, Nr3a1, ERalpha, AA420328, AU041214, ER-alpha, ER[b], Estrb, ERbeta, Esr, RNESTROR, eralpha, zfER[a], ESR2, ERB, Errra, ERRalpha, ER[b]1, zfER[b]1, MGC103724, zfER-beta1, ER[b]2, ER[b]a, zfER[b]2, zfER-beta2, erb, esrb, estrb, nr3a2, er-beta, esr-beta, XER, era, esr, esra, nr3a1, xesr-1, xlERalpha1, xlERalpha2, CERB, ER-beta, ESR1, LOC398733, LOC398734, ESRRA, ESTRRA, ERBETA, ERALPHA, err1, erra, esrral, erralpha, esrl1, nr3b1, MGC147343, er, LOC100125507

Application Details

Application Notes: Immunoprecipitation: 1:50. Western Blot: 1:50 to 1:100. Immunohistochemistry on formalin-fixed paraffin embedded sections: diluted of 1:20 to 1:80 in an ABC method (30 minutes at room temperature) Recommended Positive Control: Breast carcinoma Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Purification: Supernatant

Buffer: 10 mM citrate buffer, pH 6.0 - containing sodium azide as preservative.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Research Area: Hormones, Receptors

Restrictions: For Research Use only

Publications

Bur, Zimarowski, Schnitt et al.: "Estrogen receptor immunohistochemistry in carcinoma in situ of the breast." in: Cancer, Vol. 69, Issue 5, pp. 1174-81, 1992 (PubMed).

Graham, Jin, Lloyd: "Detection of estrogen receptor in paraffin-embedded sections of breast carcinoma by immunohistochemistry and in situ hybridization." in: The American journal of surgical pathology, Vol. 15, Issue 5, pp. 475-85, 1991 (PubMed).

Pertschuk, Kim, Nayer et al.: "Immunocytochemical estrogen and progestin receptor assays in breast cancer with monoclonal antibodies. Histopathologic, demographic, and biochemical correlations and relationship to endocrine response and survival." in: Cancer, Vol. 66, Issue 8, pp. 1663-70, 1990 (PubMed).

Kumar, Green, Stack et al.: "Functional domains of the human estrogen receptor." in: Cell, Vol. 51, Issue 6, pp. 941-51, 1988 (PubMed).